Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/347162
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | The role of CYP71A12 monooxygenase in pathogen-triggered tryptophan metabolism and Arabidopsis immunity |
Autor: | Pastorczyk, Marta; Kosaka, Ayumi; Piślewska-Bednarek, Mariola; López, Gema; Frerigmann, Henning; Kułak, Karolina; Glawischnig, Erich; Molina, Antonio; Takano, Yoshitaka; Bednarek, Paweł | Palabras clave: | Arabidopsis thaliana Camalexin Glucosinolates Indole-3-carboxylic acid Plant immunity Tryptophan metabolism |
Fecha de publicación: | 1-ene-2020 | Editor: | Blackwell Publishing | Citación: | New Phytologist 225(1): 400-412 (2020) | Resumen: | Effective defense of Arabidopsis against filamentous pathogens requires two mechanisms, both of which involve biosynthesis of tryptophan (Trp)-derived metabolites. Extracellular resistance involves products of PEN2-dependent metabolism of indole glucosinolates (IGs). Restriction of further fungal growth requires PAD3-dependent camalexin and other, as yet uncharacterized, indolics. This study focuses on the function of CYP71A12 monooxygenase in pathogen-triggered Trp metabolism, including the biosynthesis of indole-3-carboxylic acid (ICA). Moreover, to investigate the contribution of CYP71A12 and its products to Arabidopsis immunity, we analyzed infection phenotypes of multiple mutant lines combining pen2 with pad3, cyp71A12, cyp71A13 or cyp82C2. Metabolite profiling of cyp71A12 lines revealed a reduction in ICA accumulation. Additionally, analysis of mutant plants showed that low amounts of ICA can form during an immune response by CYP71B6/AAO1-dependent metabolism of indole acetonitrile, but not via IG hydrolysis. Infection assays with Plectosphaerella cucumerina and Colletotrichum tropicale, two pathogens with different lifestyles, revealed cyp71A12-, cyp71A13- and cyp82C2-associated defects associated with Arabidopsis immunity. Our results indicate that CYP71A12, but not CYP71A13, is the major enzyme responsible for the accumulation of ICA in Arabidopsis in response to pathogen ingression. We also show that both enzymes are key players in the resistance of Arabidopsis against selected filamentous pathogens after they invade. | Descripción: | 13 Pág. | Versión del editor: | https://doi.org/10.1111/nph.16118 | URI: | http://hdl.handle.net/10261/347162 | DOI: | 10.1111/nph.16118 | ISSN: | 0028-646X | E-ISSN: | 1469-8137 |
Aparece en las colecciones: | (INIA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
oficial.pdf | artículo | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
23
checked on 04-may-2024
SCOPUSTM
Citations
46
checked on 08-may-2024
WEB OF SCIENCETM
Citations
42
checked on 24-feb-2024
Page view(s)
15
checked on 07-may-2024
Download(s)
1
checked on 07-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.