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Título: | Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection |
Autor: | Méndez-López, Eduardo; Donaire, Livia CSIC ORCID ; Gosálvez, Blanca CSIC ORCID; Díaz-Vivancos, Pedro; Sánchez-Pina, M. Amelia CSIC ORCID ; Tilsner, Jens; Aranda, Miguel A. CSIC ORCID | Palabras clave: | Host factor PepMV Potexvirus Redox homeostasis Resistance Stress response Viral replication complex (VRC) |
Fecha de publicación: | abr-2023 | Editor: | New Phytologist Trust John Wiley & Sons |
Citación: | New Phytologist 238(1): 332-348 (2023) | Resumen: | Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses world-wide. No PepMV-resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV-resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase (GST) SlGSTU38 was identified in a yeast two-hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked-out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild-type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell-autonomous manner and relocalizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers reactive oxygen species accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: On the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay PepMV-infection sensing by participating in the redox intracellular homeostasis in a nonspecific manner | Versión del editor: | https://doi.org/10.1111/nph.18728 | URI: | http://hdl.handle.net/10261/340468 | DOI: | 10.1111/nph.18728 | ISSN: | 0028-646X | E-ISSN: | 1469-8137 |
Aparece en las colecciones: | (CEBAS) Artículos |
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