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Título: | Atomic crystal structure and sugar specificity of a β-trefoil lectin domain from the ectomycorrhizal basidiomycete Laccaria bicolor |
Autor: | Acebrón, Iván CSIC ORCID; Campanero-Rhodes, María Asunción CSIC ORCID; Solís, Dolores; Menéndez, Margarita CSIC ORCID; García Rodríguez, Carolina CSIC ORCID; Lillo, M. Pilar CSIC ORCID; Mancheño, Jose M. CSIC ORCID | Palabras clave: | Crystal structure Sugar-specificity at atomic resolution β-Trefoil |
Fecha de publicación: | 1-abr-2023 | Editor: | Elsevier | Citación: | International Journal of Biological Macromolecules 233: 123507 (2023) | Resumen: | Lectins from fruiting bodies are a diverse group of sugar-binding proteins from mushrooms that face the biologically relevant challenge of discriminating self- from non-self carbohydrate structures, therefore providing a basis for an innate defence system. Such a system entails both detection and destruction of invaders and/or feeders, and in contrast to more complex organisms with immense immune systems, these two functions normally rely on multitasking lectins, namely, lectins with different functional modules. Here, we present a novel fungal lectin, LBL, from the basidiomycete Laccaria bicolor. Using a diverse set of biophysical techniques, we unveil the fine details of the sugar-binding specificity of the N-terminal β-trefoil of LBL (LBL152), whose structure has been determined at the highest resolution so far reported for such a fold. LBL152 binds complex poly-N-Acetyllactosamine polysaccharides and also robust LBL152 binding to Caenorhabditis elegans and Drosophila melanogaster cellular extracts was detected in microarray assays, with a seeming preference for the fruit fly adult and pupa stages over the larva stage. Prediction of the structure of the C-terminal part of LBL with AlphaFold reveals a tandem repeat of two structurally almost identical domains of around 110 amino acids each, despite sharing low sequence conservation. | Descripción: | 17 pags., 11 figs., 3 tabs. | Versión del editor: | https://doi.org/10.1016/j.ijbiomac.2023.123507 | URI: | http://hdl.handle.net/10261/306033 | DOI: | 10.1016/j.ijbiomac.2023.123507 | ISSN: | 0141-8130 |
Aparece en las colecciones: | (IQF) Artículos |
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Atomic_crystal_structure.pdf | Artículo principal | 12,11 MB | Adobe PDF | Visualizar/Abrir |
1-s2.0-S0141813023004002-mmc1.docx | Supplementary information | 2,82 MB | Microsoft Word XML | Visualizar/Abrir |
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