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Título: | Use of enzymatic cDNA amplification as a method of detection of bean yellow mosaic virus |
Autor: | Castro, Serafina; Carazo, G.; Sáiz, Margarita CSIC ORCID; Romero, Javier CSIC; De Blas, C. | Palabras clave: | Detection Bean common mosaic virus RT-PCR Potato virus Y |
Fecha de publicación: | 1993 | Editor: | Kluwer Academic Publishers | Citación: | Netherlands Journal of Plant Pathology 99: 97-100 (1993) | Resumen: | The C-terminal region of bean yellow mosaic virus (BYMV) coat protein gene was selected for the design of oligonucleotide primers. Reverse transcription of viral RNA present in sap of virus-infected plants followed by polymerase chain reaction (RT-PCR) was performed for amplification of a 449 baise pairs fragment. These primers supported BYMV amplification, but did not support amplification on both bean common mosaic virus (BCMV) and potato virus Y (PVY) templates. © 1993 Koninklijke Nederlandse Planteziektenkundige Vereniging. | URI: | http://hdl.handle.net/10261/295165 | DOI: | 10.1007/BF01998477 | ISSN: | 0028-2944 |
Aparece en las colecciones: | (INIA) Artículos |
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