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http://hdl.handle.net/10261/294863
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Di Gaspero, G. | es_ES |
dc.contributor.author | Cipriani, G. | es_ES |
dc.contributor.author | Marrazzo, M. T. | es_ES |
dc.contributor.author | Andreetta, D. | es_ES |
dc.contributor.author | Prado, María Jesús | es_ES |
dc.contributor.author | Peterlunger, E. | es_ES |
dc.contributor.author | Testolin, R. | es_ES |
dc.date.accessioned | 2023-02-20T10:42:39Z | - |
dc.date.available | 2023-02-20T10:42:39Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Molecular Breeding 15: 11-20 (2005) | es_ES |
dc.identifier.issn | 1380-3743 | - |
dc.identifier.uri | http://hdl.handle.net/10261/294863 | - |
dc.description.abstract | Primers were developed for 118 microsatellites isolated from grape (Vitis vinifera) genomic libraries enriched for (AC)n repeats. Only one microsatellite sequence matched other grape SSR-sequences in the GeneBank database. Genotyping was carried out in the parental lines and four offspring of two pseudo-test-cross populations, 'Cabernet Sauvignon' x 'Seyval' and 'Chardonnay' x 'Bianca', and a further six other grape genotypes (V. vinifera 'Sultanina', 'Merlot', 'Syrah', 'Müller-Thurgau', Vitis 'Regent' and V. riparia 'Gloire de Montpellier'). A total of 108 microsatellites showed easily scorable alleles and 100 of them segregated according to a configuration suitable for mapping in either cross. A further 8 SSRs, although unsuitable for mapping in those crosses, showed polymorphism in the other genotypes tested. This set of markers was used, along with 75 microsatellites of other repeat-types, to fingerprint 46 offspring of the cross 'Chardonnay' x 'Bianca'. For each full-sib, individual heterozygosity and distance in repeat units between pairs of alleles at each locus (mean d 2) were calculated as a tool for predicting 'highly outbred' recombinant individuals. Six microsatellites with segregation ratios significantly distorted towards the lack of homozygous sibs were identified and mapped to linkage groups LG 3 and LG 5. Estimation of heterozygosity at genome-wide level and genotyping at loci for which homozygous sibs are discriminated against are discussed for marker-assisted background selection in outcrossing grapevines. © 2005 Springer. | es |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.relation.ispartof | Instituto de Ciencias Forestales (ICIFOR) | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | DNA markers | es |
dc.subject | Genotyping | es |
dc.subject | Inbreeding depression | es |
dc.subject | MAS | es |
dc.subject | SSR | es |
dc.title | Isolation of (AC)n-microsatellites in Vitis vinifera L.;analysis of genetic background in grapevines under marker assisted selection | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1007/s11032-004-1362-4 | - |
dc.identifier.e-issn | 1572-9788 | - |
dc.relation.csic | Sí | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
dc.issue.number | 1 | es |
dc.journal.title | Molecular Breeding | es |
dc.page.initial | 11 | es |
dc.page.final | 20 | es |
dc.volume.number | 15 | es |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
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