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Título: | Induction of anti foot and mouth disease virus T and B cell responses in cattle immunized with a peptide representing ten amino acids of VP1 |
Autor: | Zamorano, Patricia; Wigdorovitz, A.; Pérez-Filgueira, M.; Escribano, J. M.; Sadir, A. M.; Borca, M. V. | Palabras clave: | Foot and mouth disease Cattle Immune response T epitope B epitope |
Fecha de publicación: | 1998 | Editor: | Elsevier | Citación: | Vaccine 16(6): 558-563 (1998) | Resumen: | We previously demonstrated that the immunization of cattle with a synthetic peptide representing the amino acid sequence of foot and mouth disease virus (FMDV) type O1 Campos VP1 residues 135-160 (p135-160), containing immunodominant T and B epitopes, was able to induce a strong neutralizing antibody (NA) response. The epitope mapping of p135-160 identified T and B epitopes in the area restricted to amino acid residues 135-144 (Zamorano et al. 1994, Virology 201; 1995, Virology 212). We are now reporting that, although immunization with a synthetic peptide covering amino acids 135-144 (p135-144) failed to elicit an anti-FMDV response, a synthetic peptide representing a tandem duplication of the VP1 epitope 135-144 (p135-144 x 2) was very efficient in inducing a strong NA response in cattle. Both the antibody and T cell responses elicited by p135-144 x 2 were highly specific for the VP1 135-144 sequence since no reactivity was detected against synthetic peptides representing the 140-160 sequence of VP1. Additionally, both responses to B and T epitopes were long lasting in the immunized cattle. These results constitute a good example of the improvement of the immune response by rational handling of precisely identified B and T epitopes. To our knowledge, this is the shortest native amino acid sequence to induce a significant NA response to FMDV in cattle. | URI: | http://hdl.handle.net/10261/294770 | DOI: | 10.1016/S0264-410X(97)00244-2 | ISSN: | 0264-410X |
Aparece en las colecciones: | (INIA) Artículos |
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