Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/294035
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Mitochondrial genome polymorphisms associated with longissimus muscle composition in Iberian pigs |
Autor: | Fernández, Ana Isabel; Alves, Estefania; Fernández, Almudena; De Pedro, E.; López García, María Ángeles; Óvilo Martín, Cristina; Rodríguez, M. Carmen; Silió, Luis | Palabras clave: | Cytochrome c oxidase subunit III Iberian pig Intramuscular fat Protein content Mitochondrial DNA 12S rRNA |
Fecha de publicación: | 2008 | Editor: | Oxford University Press | Citación: | Journal of Animal Science 86(6): 1283-1290 (2008) | Resumen: | We carried out a study to investigate the associations between mitochondrial DNA polymorphisms and meat quality traits (intramuscular fat and protein content of the longissimus) in an Iberian porcine line named Torbiscal. The studied pigs (n = 319) belong to 9 maternal lineages and were previously assigned to 6 mitochondrial haplotypes (H1 to H6), based on Cytochrome b and Dloop sequences. Statistical analyses, following a bivariate mixed model, show a greater fat content and lower protein content in H3 haplotype carriers than H1, H2, H4, H5, and H6 haplotype carriers. The magnitudes of these differences are close to 1 g of fat and -0.5 g of protein per 100 g of muscle. To identify the causative mutation of these effects on intramuscular fat and protein contents, the complete mitochondrial DNA sequence of 6 individuals was determined, each one carrying a different mitochondrial haplotype. The alignments of these 6 complete mitochondrial sequences allowed identification of 32 substitutions and 2 indels. Two polymorphic positions were exclusively detected in H3 carriers a synonymous transition 9104C > T in the gene-coding region of Cytochrome c oxidase subunit III and a substitution 715A > G in 12S rRNA. Genotyping results of a larger number of Torbiscal samples showed the exclusive presence of 9104T and 715G alleles in H3 carriers. The detected candidate substitutions are located in essential mitochondrial genes, and although they do not change the amino acid composition, we cannot disregard a potential change in the secondary structure of their corresponding mRNA. The usefulness of these polymorphisms as markers in selection programs requires validation of the consistency of these results in other Iberian pig lines. © 2008 American Society of Animal Science. All rights reserved. | URI: | http://hdl.handle.net/10261/294035 | DOI: | 10.2527/jas.2007-0568 | ISSN: | 0021-8812 | E-ISSN: | 1525-3163 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
41
checked on 17-abr-2024
WEB OF SCIENCETM
Citations
35
checked on 25-feb-2024
Page view(s)
21
checked on 27-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.