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Título: | Molecular characterization and expression of porcine Siglec-5 |
Autor: | Escalona, Z.; Álvarez, Belén; Uenishi, H.; Toki, D.; Yuste, Miriam CSIC; Revilla Calvo, Concepción; Gómez del Moral, M.; Alonso, Fernando; Ezquerra Martínez, Ángel; Domínguez, Javier | Palabras clave: | Siglec-5 Monoclonal antibody Swine Myeloid cells Plasmacytoid DCs |
Fecha de publicación: | 2014 | Editor: | Elsevier | Citación: | Developmental and Comparative Immunology 44(1): 206-216 (2014) | Resumen: | In this study we describe the characterization of the porcine orthologue of Siglec-5. A cDNa clone was obtained from a porcine cDNa library derived from swine small intestine which encodes a 555 a-a type 1 transmembrane protein with sequence homology to human Siglec-5. This protein consists of four Ig-like domains, a transmembrane region, and a cytoplasmic tail with two tyrosine-based signalling motifs. When expressed as a recombinant protein fused to the Fc region of human IgG1, porcine Siglec-5 was able to bind porcine red blood cells in a sialic acid-dependent manner. Monoclonal antibodies (mAb) were developed against porcine Siglec-5 and used to analyse its expression in bone marrow and blood cells, and lymphoid tissues. Porcine Siglec-5 expression was mainly restricted to myelomonocytic cells and their precursors, being detected also, although at low levels, on plasmacytoid dendritic cells and B lymphocytes. In lymphoid tissues, ellipsoids of the spleen and subcapsular and medullar sinuses of lymph nodes were positive for Siglec-5. These mAbs were able to precipitate, from granulocyte lysates, a protein of approximately 85. kDa under non-reducing conditions, indicating that porcine Siglec-5 is expressed as a monomer in the plasma membrane. © 2013 Elsevier Ltd. | URI: | http://hdl.handle.net/10261/293322 | DOI: | 10.1016/j.dci.2013.12.013 | ISSN: | 0145-305X |
Aparece en las colecciones: | (INIA) Artículos |
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