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Título: | Interleukin 8 and CK-6 chemokines specifically attract rainbow trout (Oncorhynchus mykiss) RTS11 monocyte-macrophage cells and have variable effects on their immune functions |
Autor: | Montero, Jana; Coll Morales, Julio; Sevilla, Noemí CSIC ORCID ; Cuesta, Alberto; Bols, N. C.; Tafalla, Carolina CSIC ORCID | Palabras clave: | Rainbow trout Macrophages Monocytes Chemokines Interleukin 8 (IL-8) CK-6 |
Fecha de publicación: | 2008 | Editor: | Elsevier | Citación: | Developmental and Comparative Immunology 32(11): 1374-1384 (2008) | Resumen: | In the current work, we have demonstrated that both rainbow trout (Oncorhynchus mykiss) interleukin 8 (IL-8), a CXC chemokine, and CK-6, a CC chemokine, are able of efficiently attracting RTS11, a rainbow trout established macrophage-monocyte-like cell line. Interestingly, two sub-populations of non-adherent cells are distinguishable by flow cytometry that could be identified as immature monocyte- and mature macrophage-like populations, respectively, and the two chemokines studied exert their effects on different populations. Although IL-8 specifically attracts the monocyte-like sub-population, CK-6 specifically attracts the macrophage-like cell sub-population. We have also determined the effects of both of these chemokines on RTS11 phagocytosis, respiratory burst and the expression of other immune-related genes. We found that IL-8 inhibited the phagocytosis capacity of RTS11 cells belonging to the macrophage-like profile. No effect was observed, however, on the respiratory burst, immune function that has been considerably affected throughout the establishment of the cell culture. Concerning the effect that IL-8 and CK-6 have on the expression of other immune genes, we found that IL-8 significantly induced the levels of expression of CK-6, IL-8, pro-inflammatory cytokines such as IL-1β and tumour necrosis factor α (TNF-α) of RTS11 cells. On the other hand, CK-6 induced the levels of expression of IL-8, iNOS and the integrin CD-18, while it had very faint effect on pro-inflammatory cytokines. These results constitute one of the very few studies in which the effect of IL-8, a CXC chemokine, on monocyte-like cells is described. Moreover, it demonstrates that different monocyte-macrophage sub-populations have different reactivity to different chemokines. © 2008 Elsevier Ltd. All rights reserved. | URI: | http://hdl.handle.net/10261/292887 | DOI: | 10.1016/j.dci.2008.05.004 | ISSN: | 0145-305X |
Aparece en las colecciones: | (INIA) Artículos |
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