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Título: | Function of the plant DNA polymerase epsilon in replicative stress sensing, a genetic analysis |
Autor: | Pedroza-García, J. A.; Mazubert, C.; del Olmo, I.; Bourge, M.; Domenichini, S.; Bounon, R.; Tariq, Z.; Delannoy, E.; Piñeiro Galvin, Manuel Ángel; Jarillo, José Antonio; Bergounioux, C.; Benhamed, M.; Raynaud, C. | Fecha de publicación: | 2017 | Editor: | Oxford University Press | Citación: | Plant Physiology 173(3): 1735-1749 (2017) | Resumen: | Faithful transmission of the genetic information is essential in all living organisms. DNA replication is therefore a critical step of cell proliferation, because of the potential occurrence of replication errors or DNA damage when progression of a replication fork is hampered causing replicative stress. Like other types of DNA damage, replicative stress activates the DNA damage response, a signaling cascade allowing cell cycle arrest and repair of lesions. The replicative DNA polymerase « (Pol «) was shown to activate the S-phase checkpoint in yeast in response to replicative stress, but whether this mechanism functions in multicellular eukaryotes remains unclear. Here, we explored the genetic interaction between Pol « and the main elements of the DNA damage response in Arabidopsis (Arabidopsis thaliana). We found that mutations affecting the polymerase domain of Pol « trigger ATRdependent signaling leading to SOG1 activation, WEE1-dependent cell cycle inhibition, and tolerance to replicative stress induced by hydroxyurea, but result in enhanced sensitivity to a wide range of DNA damaging agents. Using knock-down lines, we also provide evidence for the direct role of Pol « in replicative stress sensing. Together, our results demonstrate that the role of Pol « in replicative stress sensing is conserved in plants, and provide, to our knowledge, the first genetic dissection of the downstream signaling events in a multicellular eukaryote. © 2017 American Society of Plant Biologists. All rights reserved. | URI: | http://hdl.handle.net/10261/292035 | DOI: | 10.1104/pp.17.00031 | ISSN: | 0032-0889 | E-ISSN: | 1532-2548 |
Aparece en las colecciones: | (INIA) Artículos |
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