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Título: | Protection against Rift Valley fever virus infection in mice upon administration of interferon-inducing RNA transcripts from the FMDV genome |
Autor: | Lorenzo, Gema CSIC ORCID ; Rodríguez Pulido, Miguel Ramón CSIC ORCID; López-Gil, E.; Sobrino Castelló, Francisco CSIC ORCID; Borrego, Belén CSIC ORCID; Sáiz, Margarita CSIC ORCID; Brun Torres, Alejandro | Palabras clave: | Rift Valley fever Foot and mouth disease virus Non coding RNA regions Innate immunity |
Fecha de publicación: | 2014 | Editor: | Elsevier | Citación: | Antiviral Research 109: 64-67 (2014) | Resumen: | In this work we have addressed the effect of synthetic, non-infectious, RNA transcripts, mimicking structural domains of the non-coding regions (NCRs) of the foot-and-mouth disease virus (FMDV) genome on the infection of mice with Rift Valley fever virus (RVFV). Groups of 5 mice were inoculated intraperitoneally (i.p.) with 200 μg of synthetic RNA resembling the 5′-terminal S region, the internal ribosome entry site (IRES) or the 3′-NCR of the FMDV genome. RNA inoculation was performed 24 h before (-24 h), 24 h after (+24 h) or simultaneously to the challenge with a lethal dose of RVFV. Administration of the IRES RNA afforded higher survival rates than administration of S or 3′NCR transcripts either at -24 h or +24 h after challenge. In contrast, when RNA inoculation and viral challenge were performed simultaneously, all mice survived in both IRES- and 3′NCR-inoculated groups, with an 80% survival in mice receiving the S RNA. Among survivors, a complete correlation between significant anti-RVFV circulating antibody titers and resistance to a second lethal challenge with the virus was observed, supporting a limited viral replication in the RNA-inoculated animals upon the first challenge. All three RNA transcripts were able to induce the production of systemic antiviral and pro-inflammatory cytokines. These data show that triggering of intracellular pathogen sensing pathways constitutes a promising approach towards development of novel RVF preventive or therapeutic strategies. © 2014 Elsevier B.V. All rights reserved. | URI: | http://hdl.handle.net/10261/291584 | DOI: | 10.1016/j.antiviral.2014.06.010 | ISSN: | 0166-3542 | E-ISSN: | 1872-9096 |
Aparece en las colecciones: | (INIA) Artículos |
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