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Título: | Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins |
Autor: | Gonzalez Barderas, María; Rodríguez, Fernando; Gómez-Puertas, Paulino CSIC ORCID ; Avilés, Manuel; Beitia, F.; Alonso Martí, Covadonga; Escribano, J. M. | Fecha de publicación: | 2001 | Editor: | Springer Nature | Citación: | Archives of Virology 146: 1681-1691 (2001) | Resumen: | A chimera of the two immunodominant African swine fever (ASF) virus proteins p54 and p30 was constructed by insertion of the gene CP204L into a Not I restriction site of E183L gene. The resulting chimeric protein p54/30, expressed by a recombinant baculovirus in insect cells and in Trichoplusia ni larvae, retained antigenic determinants present in both proteins and reacted in Western blot with a collection of sera from inapparent ASF virus carrier pigs. Remarkably, pigs immunized with the chimeric protein developed neutralizing antibodies and survived the challenge with a virulent African swine fever virus, presenting a reduction of about two logs in maximum viremia titers with respect to control pigs. In conclusion, this study revealed that the constructed chimeric protein may have utility as a serological diagnostic reagent and for further immunological studies that may provide new insights on mechanisms of protective immunity to ASFV. | URI: | http://hdl.handle.net/10261/291391 | DOI: | 10.1007/s007050170056 | ISSN: | 0304-8608 | E-ISSN: | 1432-8798 |
Aparece en las colecciones: | (INIA) Artículos |
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