Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/291032
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Infectious pancreatic necrosis virus triggers antiviral immune response in rainbow trout red blood cells, despite not being infective |
Autor: | Ortega-Villaizán, M. M.; Nombela, Iván; Carrion, A.; Puente-Marín, Sara; Chico, Verónica; Mercado, Luis; Pérez, L.; Coll Morales, Julio | Palabras clave: | Erythrocytes IPNV Birnavirus Immune response Antiviral Trout Interferon |
Fecha de publicación: | 2017 | Editor: | F1000Research | Citación: | F1000Research 6: e1968 (2017) | Resumen: | Background: Some fish viruses, such as piscine orthoreovirus and infectious salmon anemia virus, target red blood cells (RBCs), replicate inside them and induce an immune response. However, the roles of RBCs in the context of infectious pancreatic necrosis virus (IPNV) infection have not been studied yet. Methods: Ex vivo rainbow trout RBCs were obtained from peripheral blood, Ficoll purified and exposed to IPNV in order to analyze infectivity and immune response using RT-qPCR, immune fluorescence imaging, flow cytometry and western-blotting techniques. Results: IPNV could not infect RBCs; however, IPNV increased the expression of the INF1-related genes ifn-1, pkr and mx genes. Moreover, conditioned media from IPNV-exposed RBCs conferred protection against IPNV infection in CHSE-214 fish cell line. Conclusions: Despite not being infected, rainbow trout RBCs could respond to IPNV with increased expression of antiviral genes. Fish RBCs could be considered as mediators of the antiviral response and therefore targets of new strategies against fish viral infections. Further research is ongoing to completely understand the molecular mechanism that triggers this antiviral response in rainbow trout RBCs. | URI: | http://hdl.handle.net/10261/291032 | DOI: | 10.12688/f1000research.12994.2 | ISSN: | 2046-1402 |
Aparece en las colecciones: | (INIA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
242.PDF | 1,11 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
28
checked on 29-mar-2024
SCOPUSTM
Citations
39
checked on 23-abr-2024
Page view(s)
34
checked on 27-abr-2024
Download(s)
19
checked on 27-abr-2024