Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/290276
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Sensitive detection of African swine fever virus using real-time PCR with a 5' conjugated minor groove binder probe |
Autor: | McKillen, J.; McMenamy, M.; Hjertner, B.; McNeilly, F.; Uttenthal, Å.; Gallardo, Carmina; Adair, B.; Allan, G. | Palabras clave: | African swine fever virus Real-time PCR Minor groove binder probe |
Fecha de publicación: | 2010 | Editor: | Elsevier | Citación: | Journal of Virological Methods 168(1-2): 141-146 (2010) | Resumen: | The design of a 5' conjugated minor groove binder (MGB) probe real-time PCR assay is described for the rapid, sensitive and specific detection of African swine fever virus (ASFV) DNA. The assay is designed against the 9GL region and is capable of detecting 20 copies of a DNA standard. It does not detect any of the other common swine DNA viruses tested in this study. The assay can detect ASFV DNA in a range of clinical samples. Sensitivity was equivalent to the Office International des Epizooties (OIE) recommended TaqMan assay. In addition the assay was found to have a detection limit 10-fold more sensitive than the conventional PCR recommended by the OIE. Linear range was ten logs from 2×101 to 2×1010. The assay is rapid with an amplification time just over 2h. The development of this assay provides a useful tool for the specific diagnosis of ASF in statutory or emergency testing programs or for the detection of ASFV DNA in research applications. © 2010 Elsevier B.V. | URI: | http://hdl.handle.net/10261/290276 | DOI: | 10.1016/j.jviromet.2010.05.005 | ISSN: | 0166-0934 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
31
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
28
checked on 24-feb-2024
Page view(s)
14
checked on 27-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.