Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/289832
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection in pigs |
Autor: | García-Roselló, E.; Matás, Carmen; Cánovas, Sebastián; Moreira, Pedro Nuno; Gadea, J.; Coy, P. | Palabras clave: | Capacitation Sperm cryopreservation Acrosome reaction Early development |
Fecha de publicación: | 2006 | Editor: | John Wiley & Sons | Citación: | Journal of Andrology 27(2): 268-275 (2006) | Resumen: | The purpose of this study was to determine the influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection (ICSI) in pigs. This was done by examining the effect of 1) the conservation method (fresh vs frozen); 2) the sperm treatment preinjection (resuspension in Dulbecco phosphate-buffered saline (DPBS) vs selection by a Percoll gradient); and 3) the acrosomal and live or dead status of the spermatozoa (by incubation with or without calcium ionophore, 1 μM and 5 μM). In vitro matured porcine oocytes were injected with treated spermatozoa according to each experiment. All the experiments were done with non-artificially activated oocytes. The percentages of activation and cleavage were higher (68% vs 43% and 63% vs 43%, respectively, P < .05) in oocytes injected with fresh vs frozen spermatozoa. The DPBS treatment allowed higher cleavage proportions than the Percoll treatment (P < .05). Moreover, a boar effect was observed in the percentage of developing blastocysts. None of the studied parameters was affected by the acrosomal or the live or dead status of the spermatozoa injected. In conclusion, the use of fresh semen is recommended for porcine ICSI, as well as careful selection of the boar; Percoll treatment is only recommended for poor-quality samples or for removing toxic agents, and no exogenous form of activation or induction of the acrosome reaction is necessary for porcine oocytes to develop a male pronucleus and cleave up to the 2-cell stage after ICSI, although experimental conditions to reach the blastocyst stage need to be investigated further. Copyright © American Society of Andrology. | URI: | http://hdl.handle.net/10261/289832 | DOI: | 10.2164/jandrol.05110 | E-ISSN: | 1939-4640 |
Aparece en las colecciones: | (INIA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
24
checked on 15-may-2024
WEB OF SCIENCETM
Citations
22
checked on 28-feb-2024
Page view(s)
28
checked on 16-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.