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Título: | Use of Foot-and-Mouth Disease Virus Non-coding Synthetic RNAs as Vaccine Adjuvants |
Otros títulos: | Methods and Protocols | Autor: | Rodríguez Pulido, Miguel Ramón CSIC ORCID; Polo, Miryam; Borrego, Belén CSIC ORCID; Sáiz, Margarita CSIC ORCID | Palabras clave: | Foot-and-mouth disease vaccines; Foot-and-mouth disease virus; Immunomodulatory RNA RNA delivery Non-coding RNA |
Fecha de publicación: | 3-feb-2022 | Editor: | Springer Nature | Citación: | Vaccine Technologies for Veterinary Viral Diseases: 306(2022) | Serie: | Methods in Molecular Biology 2465 |
Resumen: | The ncRNAs are short RNA transcripts with sequence and structure resembling that of specific domains in the non-coding regions of the foot-and-mouth disease (FMD) virus (FMDV ) genome. These synthetic molecules induce a robust antiviral response and have been shown to enhance the immune response and protection induced by an FMD inactivated vaccine in pigs. Here, we describe the method for ncRNAs synthesis, formulation, and delivery into mice and pigs for studies focused on testing the adjuvant effect of RNA-based strategies in combination with veterinarian vaccines. | Descripción: | 125-136 pp | Versión del editor: | https://doi.org/10.1007/978-1-0716-2168-4 | URI: | http://hdl.handle.net/10261/274627 | DOI: | 10.1007/978-1-0716-2168-4_7 | ISBN: | 978-1-0716-2167-7 978-1-0716-2168-4 (eBook) |
ISSN: | 1064-3745 | E-ISSN: | 1940-6029 |
Aparece en las colecciones: | (INIA) Libros y partes de libros |
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FICHERO COMODÍN.pdf | Capítulo de libro | 59,24 kB | Adobe PDF | Visualizar/Abrir |
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