Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/270796
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose |
Autor: | Bisquert, Ricardo CSIC ORCID; Planells-Cárcel, Andrés CSIC; Valera García, Elena CSIC; Guillamón, José Manuel CSIC ORCID ; Muñiz Calvo, Sara CSIC ORCID | Palabras clave: | Hydroxytyrosol Antioxidants HpaBC |
Fecha de publicación: | 24-oct-2021 | Editor: | Society for Applied Microbiology | Citación: | Microbial Biotechnology 15 (5):1499-1510 (2022) | Resumen: | Hydroxytyrosol (HT) is one of the most powerful dietary antioxidants with numerous applications in different areas, including cosmetics, nutraceuticals and food. In the present work, heterologous hydroxylase complex HpaBC from Escherichia coli was integrated into the Saccharomyces cerevisiae genome in multiple copies. HT productivity was increased by redirecting the metabolic flux towards tyrosol synthesis to avoid exogenous tyrosol or tyrosine supplementation. After evaluating the potential of our selected strain as an HT producer from glucose, we adjusted the medium composition for HT production. The combination of the selected modifications in our engineered strain, combined with culture conditions optimization, resulted in a titre of approximately 375 mg l-1 of HT obtained from shake-flask fermentation using a minimal synthetic-defined medium with 160 g l-1 glucose as the sole carbon source. To the best of our knowledge, this is the highest HT concentration produced by an engineered S. cerevisiae strain. | Versión del editor: | https://doi.org/10.1111/1751-7915.13957 | URI: | http://hdl.handle.net/10261/270796 | DOI: | 10.1111/1751-7915.13957 |
Aparece en las colecciones: | (IATA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
MicrBiotech2022-Bisquert.pdf | Artículo principal | 428,48 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
7
checked on 19-abr-2024
SCOPUSTM
Citations
16
checked on 28-abr-2024
WEB OF SCIENCETM
Citations
15
checked on 28-feb-2024
Page view(s)
61
checked on 30-abr-2024
Download(s)
122
checked on 30-abr-2024