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http://hdl.handle.net/10261/268449
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Albuquerque, André | es_ES |
dc.contributor.author | Óvilo Martín, Cristina | es_ES |
dc.contributor.author | Núñez, Yolanda | es_ES |
dc.contributor.author | Benítez Yáñez, Rita María | es_ES |
dc.contributor.author | López-García, Adrián | es_ES |
dc.contributor.author | Suárez García, Fabián | es_ES |
dc.contributor.author | Félix, Maria do Rosário | es_ES |
dc.contributor.author | Laranjo, Marta | es_ES |
dc.contributor.author | Charneca, Rui | es_ES |
dc.contributor.author | Martins, José Manuel | es_ES |
dc.date.accessioned | 2022-05-03T09:21:55Z | - |
dc.date.available | 2022-05-03T09:21:55Z | - |
dc.date.issued | 2021-05-16 | - |
dc.identifier.citation | Animals 11(6):1423 (2021) | es_ES |
dc.identifier.issn | 2076-2615 | - |
dc.identifier.uri | http://hdl.handle.net/10261/268449 | - |
dc.description | 21 Págs. This article belongs to the Special Issue Transcriptomics in Autochthonous Breeds or Populations of Livestock and Aquaculture Species. | es_ES |
dc.description.abstract | Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14's coding product NIK, failing to restore insulin sensitivity. | es_ES |
dc.description.sponsorship | This work was funded by European Union’s H2020 RIA program (grant agreement no. 634476), by Portuguese national funds through FCT/MCTES under project UIDB/05183/2020, and a research grant SFRH/BD/132215/2017 for A. Albuquerque. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/634476/DIVERSITY OF LOCAL PIG BREEDS AND PRODUCTION SYSTEMS FOR HIGH QUALITY TRADITIONAL PRODUCTS AND SUSTAINABLE PORK CHAINS | es_ES |
dc.relation | SFRH/BD/132215/2017/Portuguese national funds | es_ES |
dc.relation.ispartof | Animals : an open access journal from MDPI | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Alentejano pig | es_ES |
dc.subject | Bísaro pig | es_ES |
dc.subject | MAP3K14 | es_ES |
dc.subject | MYH3 | es_ES |
dc.subject | MYH7 | es_ES |
dc.subject | Intramuscular fat | es_ES |
dc.subject | Meat quality | es_ES |
dc.subject | Skeletal muscle | es_ES |
dc.subject | Transcriptomes | es_ES |
dc.title | Transcriptomic Profiling of Skeletal Muscle Reveals Candidate Genes Influencing Muscle Growth and Associated Lipid Composition in Portuguese Local Pig Breeds | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.3390/ani11051423 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ani11051423 | es_ES |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.contributor.funder | European Commission | es_ES |
dc.contributor.funder | Fundação para a Ciência e a Tecnologia (Portugal) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100001871 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.contributor.orcid | Albuquerque, André [0000-0002-1189-9803] | es_ES |
dc.contributor.orcid | Óvilo, Cristina [0000-0002-5738-8435] | es_ES |
dc.contributor.orcid | Núñez, Yolanda [0000-0001-5988-7628] | es_ES |
dc.contributor.orcid | Benítez, Rita [0000-0001-7459-2836] | es_ES |
dc.contributor.orcid | López-Garcia, Adrián [0000-0002-8649-750X] | es_ES |
dc.contributor.orcid | Suárez García, Fabián [0000-0002-1970-293X] | es_ES |
dc.contributor.orcid | Laranjo, Marta [0000-0002-3900-5592] | es_ES |
dc.contributor.orcid | Charneca, Rui [0000-0002-3597-6746] | es_ES |
dc.contributor.orcid | Martins, José Manuel [0000-0003-3616-8406] | es_ES |
dc.identifier.pmid | 34065673 | - |
dc.identifier.scopus | 2-s2.0-85105703474 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85105703474 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (INIA) Artículos |
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Transcriptomic-Profiling-of-Skeletal.pdf | artículo | 6,65 MB | Adobe PDF | Visualizar/Abrir |
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