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http://hdl.handle.net/10261/215201
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Conlon, J. Michael | es_ES |
dc.contributor.author | Attoub, Samir | es_ES |
dc.contributor.author | Musale, Vishal | es_ES |
dc.contributor.author | Leprince, Jérôme | es_ES |
dc.contributor.author | Casewell, Nicholas R. | es_ES |
dc.contributor.author | Sanz, Libia | es_ES |
dc.contributor.author | Calvete, Juan J. | es_ES |
dc.date.accessioned | 2020-06-25T07:58:11Z | - |
dc.date.available | 2020-06-25T07:58:11Z | - |
dc.date.issued | 2020-03-18 | - |
dc.identifier.citation | Toxicon: X 6:100030 (2020) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/215201 | - |
dc.description | 8 páginas, 4 figuras, 1 tabla | es_ES |
dc.description.abstract | Four peptides with cytotoxic activity against BRIN-BD11 rat clonal β-cells were purified from the venom of the black-necked spitting cobra Naja nigricollis using reversed-phase HPLC. The peptides were identified as members of the three-finger superfamily of snake toxins by ESI-MS/MS sequencing of tryptic peptides. The most potent peptide (cytotoxin-1N) showed strong cytotoxic activity against three human tumor-derived cell lines (LC50 = 0.8 ± 0.2 μM for A549 non-small cell lung adenocarcinoma cells; LC50 = 7 ± 1 μM for MDA-MB-231 breast adenocarcinoma cells; and LC50 = 9 ± 1 μM for HT-29 colorectal adenocarcinoma cells). However, all the peptides were to varying degrees cytotoxic against HUVEC human umbilical vein endothelial cells (LC50 in the range 2-22 μM) and cytotoxin-2N was moderately hemolytic (LC50 = 45 ± 3 μM against mouse erythrocytes). The lack of differential activity against cells derived from non-neoplastic tissue limits their potential for development into anti-cancer agents. In addition, two proteins in the venom, identified as isoforms of phospholipase A2, effectively stimulated insulin release from BRIN-BD11 cells (an approximately 6-fold increase in rate compared with 5.6 mM glucose alone) at a concentration (1 μM) that was not cytotoxic to the cells suggesting possible application in therapy for Type 2 diabetes. | es_ES |
dc.description.sponsorship | This work was partly supported by the Ministerio de Ciencia, Innovacion � y Universidades, Madrid, Spain (grant number BFU 2017- 89103-P) to JJC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-89103-P | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Cytotoxicity | es_ES |
dc.subject | Insulinotropic activity | es_ES |
dc.subject | Naja | es_ES |
dc.subject | Phospholipase A2 | es_ES |
dc.subject | Three-finger toxins | es_ES |
dc.title | Isolation and characterization of cytotoxic and insulin-releasing components from the venom of the black-necked spitting cobra Naja nigricollis (Elapidae) | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.toxcx.2020.100030 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.toxcx.2020.100030 | es_ES |
dc.identifier.e-issn | 2590-1710 | - |
dc.rights.license | http://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.pmid | 32550585 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
Aparece en las colecciones: | (IBV) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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2020 Toxicon X 6-100030.pdf | 1,33 MB | Adobe PDF | Visualizar/Abrir |
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