Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/196762
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Bioaugmentation of PAH-Contaminated Soils With Novel Specific Degrader Strains Isolated From a Contaminated Industrial Site. Effect of Hydroxypropyl-β-Cyclodextrin as PAH Bioavailability Enhancer |
Autor: | Villaverde Capellán, J. CSIC ORCID ; Laiz Trobajo, L. CSIC ORCID; Lara Moreno, Alba CSIC ORCID; González-Pimentel, José Luis CSIC ORCID CVN; Morillo González, Esmeralda CSIC ORCID | Palabras clave: | Achromobacter xylosoxidans Bioaugmentation Cyclodextrin PAHs Soil Stenotrophomonas maltophilia |
Fecha de publicación: | 14-nov-2019 | Editor: | Frontiers Media | Citación: | Frontiers in Microbiology (10): 2588 (2019) | Resumen: | A PAHs-contaminated industrial soil was analyzed using PCR amplification of the gene 16S ribosomal RNA for the detection and identification of different isolated bacterial strains potentially capable of degrading PAHs. Novel degrader strains were isolated and identified as Achromobacter xylosoxidans 2BC8 and Stenotrophomonas maltophilia JR62, which were able to degrade PYR in solution, achieving a mineralization rate of about 1% day–1. A. xylosoxidans was also able to mineralize PYR in slurry systems using three selected soils, and the total extent of mineralization (once a plateau was reached) increased 4.5, 21, and 57.5% for soils LT, TM and CR, respectively, regarding the mineralization observed in the absence of the bacterial degrader. Soil TM contaminated with PYR was aged for 80 days and total extent of mineralization was reduced (from 46 to 35% after 180 days), and the acclimation period increased (from 49 to 79 days). Hydroxypropyl-ß-cyclodextrin (HPBCD) was used as a bioavailability enhancer of PYR in this aged soil, provoking a significant decrease in the acclimation period (from 79 to 54 days) due to an increase in PYR bioavailable fraction just from the beginning of the assay. However, a similar global extension of mineralization was obtained. A. xylosoxidans was then added together with HPBCD to this aged TM soil contaminated with PYR, and the total extent of mineralization decreased to 25% after 180 days, possibly due to the competitive effect of endogenous microbiota and the higher concentration of PYR in the soil solution provoked by the addition of HPBCD, which could have a toxic effect on the A. xylosoxidans strain. © Copyright © 2019 Villaverde, Láiz, Lara-Moreno, González-Pimentel and Morillo. | Descripción: | 16 páginas.- 5 graficas.- 6 tablas.- 91 referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.02588/full#supplementary-material | Versión del editor: | http://dx.doi.org/10.3389/fmicb.2019.02588 | URI: | http://hdl.handle.net/10261/196762 | DOI: | 10.3389/fmicb.2019.02588 | ISSN: | 1664-302X |
Aparece en las colecciones: | (IRNAS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Bioaugmentation_PAH_contaminated_soils_novel_2019_OA_CC4.pdf | 1,77 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
2
checked on 02-may-2024
SCOPUSTM
Citations
25
checked on 15-may-2024
WEB OF SCIENCETM
Citations
19
checked on 20-feb-2024
Page view(s)
373
checked on 15-may-2024
Download(s)
195
checked on 15-may-2024