Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/185987
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Disruption of ku70 involved in non-homologous end-joining facilitates homologous recombination but increases temperature sensitivity in the phytopathogenic fungus Penicillium digitatum |
Autor: | Gandía Gómez, Mónica CSIC ORCID; Xu, Shaomei; Font, Cristina; Marcos López, José Francisco CSIC ORCID | Palabras clave: | Citrus fruit Gene targeting Chitin synthase Fungal transformation |
Fecha de publicación: | 27-nov-2015 | Editor: | Elsevier | Citación: | Fungal Biology 120 (3): 317-323 (2016) | Resumen: | The dominant mechanism to repair double-stranded DNA breaks in filamentous fungi is the non-homologous end joining (NHEJ) pathway, and not the homologous recombination (HR) pathway that operates in the mutation of genes by replacement of target DNA for selection cassettes. The key to improve HR frequency is the inactivation of the NHEJ pathway by eliminating components of its Ku70/80 heterodimeric complex. We have obtained ku70 mutants of Penicillium digitatum, the main citrus postharvest pathogen. The increased efficiency of HR in Δku70 strains was demonstrated by the generation of mutants in two different chitin synthase genes (PdchsII and PdchsV). P. digitatum Δku70 strains showed no differences from the parental strain in vegetative growth, asexual development or virulence to citrus fruit, when experiments were conducted at the optimal temperature of 24 °C. However, growth of Δku70 strains at temperatures higher than 24 °C demonstrated a detrimental effect in axenic growth and conidia production. These observations are in agreement with previous studies describing differences between ku70 mutants and their parental strains in some fungal species, and must be taken into account for future applications of the Δku approach to increase HR efficiency in fungi. | Versión del editor: | http://dx.doi.org/10.1016/j.funbio.2015.11.001 | URI: | http://hdl.handle.net/10261/185987 | DOI: | 10.1016/j.funbio.2015.11.001 | ISSN: | 1878-6146 |
Aparece en las colecciones: | (IATA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
FB2015-Gandia.pdf | Artículo principal | 467,5 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
27
checked on 07-may-2024
WEB OF SCIENCETM
Citations
21
checked on 27-feb-2024
Page view(s)
237
checked on 07-may-2024
Download(s)
336
checked on 07-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons