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http://hdl.handle.net/10261/184945
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Araujo Farias, Gabriela de | es_ES |
dc.contributor.author | Olmedilla, Adela | es_ES |
dc.contributor.author | Gallegos, María Trinidad | es_ES |
dc.date.accessioned | 2019-06-27T08:01:08Z | - |
dc.date.available | 2019-06-27T08:01:08Z | - |
dc.date.issued | 2019 | - |
dc.identifier | doi: 10.1111/1751-7915.13385 | - |
dc.identifier | issn: 1751-7915 | - |
dc.identifier.citation | Microbial Biotechnology (2019) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/184945 | - |
dc.description.abstract | Cellulose, whose production is controlled by c-di-GMP, is a commonly found exopolysaccharide in bacterial biofilms. Pseudomonas syringae pv. tomato (Pto) DC3000, a model organism for molecular studies of plant–pathogen interactions, carries the wssABCDEFGHI operon for the synthesis of acetylated cellulose. The high intracellular levels of the second messenger c-di-GMP induced by the overexpression of the heterologous diguanylate cyclase PleD stimulate cellulose production and enhance air–liquid biofilm (pellicle) formation. To characterize the mechanisms involved in Pto DC3000 pellicle formation, we studied this process using mutants lacking flagella, biosurfactant or different extracellular matrix components, and compared the pellicles produced in the absence and in the presence of PleD. We have discovered that neither alginate nor the biosurfactant syringafactin are needed for their formation, whereas cellulose and flagella are important but not essential. We have also observed that the high c-di-GMP levels conferred more cohesion to Pto cells within the pellicle and induced the formation of intracellular inclusion bodies and extracellular fibres and vesicles. Since the pellicles were very labile and this greatly hindered their handling and processing for microscopy, we have also developed new methods to collect and process them for scanning and transmission electron microscopy. These techniques open up new perspectives for the analysis of fragile biofilms in other bacterial strains. | - |
dc.description.sponsorship | This research was supported by grants BIO2014-55075-P and BIO2017-83533-P from the Spanish MINECO/MICINN and P10-CVI-5800 from the Junta de Andalucía, all of them co-financed by European Regional Development Fund. G.A.F. was supported by a Ciencia sem Fronteiras fellowship (BEX10043/13-6) from the CAPES-Brazil. | - |
dc.publisher | John Wiley & Sons | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-55075-P; /BIO2017-83533 | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Visualization and characterization of Pseudomonas syringae pv. tomato DC3000 pellicles | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1111/1751-7915.13385 | - |
dc.relation.publisherversion | https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/1751-7915.13385 | - |
dc.date.updated | 2019-06-27T08:01:08Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Fundaçao Capes (Brasil) | - |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.identifier.pmid | 30838765 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (EEZ) Artículos |
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Fichero | Descripción | Tamaño | Formato | |
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2019_Farias_MB_OA.pdf | 4,75 MB | Adobe PDF | Visualizar/Abrir |
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