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http://hdl.handle.net/10261/18345
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dc.contributor.author | Martí, Daniel | - |
dc.contributor.author | Miquel, Raquel | - |
dc.contributor.author | Ziani, Khalid | - |
dc.contributor.author | Gisbert, Regina | - |
dc.contributor.author | Ivorra, M. Dolores | - |
dc.contributor.author | Anselmi, Elsa | - |
dc.contributor.author | Moreno, Lucrecia | - |
dc.contributor.author | Villagrasa, Victoria | - |
dc.contributor.author | Barettino, Domingo | - |
dc.contributor.author | D'Ocón, Pilar | - |
dc.date.accessioned | 2009-11-05T12:50:17Z | - |
dc.date.available | 2009-11-05T12:50:17Z | - |
dc.date.issued | 2005-06-10 | - |
dc.identifier.citation | American Journal of Physiology Heart and Circulatory Physiology. 2005 Nov;289(5):H1923-H1932 | en_US |
dc.identifier.issn | 0363-6135 (print) | - |
dc.identifier.uri | http://hdl.handle.net/10261/18345 | - |
dc.description | 10 pages, 3 figures, 10 tables.-- PMID: 15951348 [PubMed] | en_US |
dc.description.abstract | The mRNA levels for the three alpha1-adrenoceptor subtypes, alpha1A, alpha1B, and alpha1D, were quantified by real-time RT-PCR in arteries from Wistar rats. The alpha1D-adrenoceptor was prominent in both aorta (79.0%) and mesenteric artery (68.7%), alpha1A predominated in tail (61.7%) and small mesenteric artery (73.3%), and both alpha1A- and alpha1D-subtypes were expressed at similar levels in iliac artery. The mRNA levels of the alpha1B-subtype were a minority in all vessels (1.7-11.1%). Concentration-response curves of contraction in response to phenylephrine or relaxation in response to alpha1-adrenoceptor antagonists on maximal sustained contraction induced by phenylephrine were constructed from control vessels and vessels pretreated with 100 micromol/l chloroethylclonidine (CEC) for 30 min. The significant decrease in the phenylephrine potency observed after CEC treatment together with the inhibitory potency displayed by 8-{2-[4-(2-methoxyphenyl)-1-piperazinyl]-8-azaspiro (4,5) decane-7-dionedihydrochloride} (BMY-7378, an alpha1D-adrenoceptor antagonist) confirm the relevant role of alpha1D-adrenoceptors in aorta and iliac and proximal mesenteric arteries. The potency of 5-methylurapidil (an alpha1A-adrenoceptor antagonist) and the changes in the potency of both BMY-7378 and 5-methylurapidil after CEC treatment provided evidence of a mixed population of alpha1A- and alpha1D-adrenoceptors in iliac and distal mesenteric arteries. The low potency of prazosin (pIC50 < 9) as well as the high 5-methylurapidil potency in tail and small mesenteric arteries suggest the main role of alpha1A/alpha1L-adrenoceptors with minor participation of the alpha1D-subtype. The mRNA levels and CEC treatment corroborated this pattern and confirmed that the alpha1L-adrenoceptor could be a functional isoform of the alpha1A-subtype. | en_US |
dc.description.sponsorship | This work was supported by a research grant from the Spanish Comisión Interministerial de Ciencia y Tecnología (SAF2001-2656). Daniel Martí Canet received a fellowship from Universidad Cardenal Herrera-CEU. | en_US |
dc.format.extent | 15595 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | American Physiological Society | en_US |
dc.rights | closedAccess | en_US |
dc.subject | alpha1A- alpha1B alpha1D adrenoceptors | en_US |
dc.subject | Chloroethylclonidine | en_US |
dc.title | Correlation between mRNA levels and functional role of 1-adrenoceptor subtypes in arteries: evidence of 1L as a functional isoform of the 1A-adrenoceptor | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1152/ajpheart.00288.2005 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1152/ajpheart.00288.2005 | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
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