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http://hdl.handle.net/10261/170895
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Prada‐Ramírez, Harold A. | - |
dc.contributor.author | Pérez-Mendoza, Daniel | - |
dc.contributor.author | Felipe, Antonia | - |
dc.contributor.author | Martínez-Granero, Francisco | - |
dc.contributor.author | Rivilla, Rafael | - |
dc.contributor.author | Sanjuán, Juan | - |
dc.contributor.author | Gallegos, María Trinidad | - |
dc.date.accessioned | 2018-10-10T11:10:24Z | - |
dc.date.available | 2018-10-10T11:10:24Z | - |
dc.date.issued | 2016 | - |
dc.identifier | doi: 10.1111/mmi.13278 | - |
dc.identifier | issn: 1365-2958 | - |
dc.identifier.citation | Molecular Microbiology 99: 960- 977 (2016) | - |
dc.identifier.uri | http://hdl.handle.net/10261/170895 | - |
dc.description.abstract | In Pseudomonas syringae pv. tomato DC3000, the second messenger c-di-GMP has been previously shown to stimulate pellicle formation and cellulose biosynthesis. A screen for genes involved in cellulose production under high c-di-GMP intracellular levels led to the identification of insertions in two genes, wssB and wssE, belonging to the Pto DC3000 cellulose biosynthesis operon wssABCDEFGHI. Interestingly, beside cellulose-deficient mutants, colonies with a rougher appearance than the wild type also arouse among the transposants. Those mutants carry insertions in amrZ, a gene encoding a transcriptional regulator in different Pseudomonas. Here, we provide evidence that AmrZ is involved in the regulation of bacterial cellulose production at transcriptional level by binding to the promoter region of the wssABCDEFGHI operon and repressing cellulose biosynthesis genes. Mutation of amrZ promotes wrinkly colony morphology, increased cellulose production and loss of motility in Pto DC3000. AmrZ regulon includes putative c-di-GMP metabolising proteins, like AdcA and MorA, which may also impact those phenotypes. Furthermore, an amrZ but not a cellulose-deficient mutant turned out to be impaired in pathogenesis, indicating that AmrZ is a key regulator of Pto DC3000 virulence probably by controlling bacterial processes other than cellulose production. | - |
dc.description.sponsorship | We thank Sheng Yang He for the Pto fliC mutant and Gabriela A. Farias for helping with flagella staining and microscope observation. This research was supported by grants BIO2011-23032 and BIO2014-55075-P from the Spanish Ministerio de Economía y Competitividad (MINECO) and P10-CVI-5800 from the Junta de Andalucía, all of them cofi- nanced by European Regional Development Fund. H.A.P.R. was supported by a FPI fellowship from the Spanish MINECO, also cofinanced by European Regional Develop- ment Fund. D.P.M. was initially supported by a JAE-doc CSIC contract and later by contracts from grants P10-CVI-5800 and 201440E026, and by Andalucía Talent Hub Program launched by the Andalusian Knowledge Agency, cofunded by the European Union’s Seventh Framework Program, Marie Skłodowska-Curie actions (COFUND – Grant Agreement n° 291780) and Junta de Andalucía. | - |
dc.publisher | Blackwell Publishing | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-55075-P | - |
dc.rights | openAccess | - |
dc.title | AmrZ regulates cellulose production in Pseudomonas syringae pv. tomato DC3000 | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1111/mmi.13278 | - |
dc.date.updated | 2018-10-10T11:10:26Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Junta de Andalucía | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100011011 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
Aparece en las colecciones: | (EEZ) Artículos |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir | |
2016_Prada_MM.pdf | 2,08 MB | Adobe PDF | Visualizar/Abrir |
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