Construction of a high-density integrated DArTseq SNP-based genetic map and identification of genomic regions controlling rust resistance in Pisum fulvum

Abstract

The fungus Uromyces pisi, causing pea rust, is one of the major limiting factors in pea production worldwide to which limited resistance is available. We searched in previous years for resistance in a large germplasm Pisum spp. collection and failed to identify complete resistance, being the higher levels of incomplete resistance identified in P. fulvum. To unravel the genetic control underlying resistance to U. pisi in P. fulvum we generated a recombinant inbred line (RIL) population from a cross between resistant and susceptible P. fulvum accessions, which was genotyped on the Diversity Arrays Technology Pty Ltd platform. A total of 9,569 high-quality DArT-Seq and 8,514 SNPs markers were generated that assembled in seven linkage groups. The newly constructed integrated genetic linkage map of P. fulvum and the QTLs associated with rust resistance both in seedlings and adult plants will be presented.