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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/157966
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dc.contributor.authorFox, Ana Rominaes_ES
dc.contributor.authorMercado-Blanco, Jesúses_ES
dc.contributor.authorAyub, Nicolás Danieles_ES
dc.date.accessioned2017-12-04T13:44:20Z-
dc.date.available2017-12-04T13:44:20Z-
dc.date.issued2016-10-
dc.identifier.citationEnvironmental Microbiology 18(10): 3522-3534 (2016)es_ES
dc.identifier.issn1462-2912-
dc.identifier.urihttp://hdl.handle.net/10261/157966-
dc.descriptionFox, Ana Romina et al.-- Special Issue on Ecophysiology of Pseudomonas .es_ES
dc.description.abstractA main goal of biological nitrogen fixation research has been to expand the nitrogen-fixing ability to major cereal crops. In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNF to maize and wheat under non-gnotobiotic conditions. Inoculation of maize and wheat with Pf-5 X940 largely improved nitrogen content and biomass accumulation in both vegetative and reproductive tissues, and this beneficial effect was positively associated with high nitrogen fixation rates in roots. 15N isotope dilution analysis showed that maize and wheat plants obtained substantial amounts of fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolated from the maize and wheat root surface but never from the inner root tissues. Confocal laser scanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants, and microcolonies were mostly visualized at the junctions between epidermal root cells. Genetic analysis using biofilm formation-related Pseudomonas mutants confirmed the relevance of bacterial root adhesion in the increase in nitrogen content, biomass accumulation and nitrogen fixation rates in wheat roots. To our knowledge, this is the first report of robust BNF in major cereal crops.es_ES
dc.description.sponsorshipThis work was supported by grants PICT-2011-1325, PICT-2014-1397 and PICT-2014-3659 to N.D.A.es_ES
dc.language.isoenges_ES
dc.publisherWiley-Blackwelles_ES
dc.relation.isversionofPostprint-
dc.rightsopenAccesses_ES
dc.titleMajor cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940es_ES
dc.typeartículoes_ES
dc.identifier.doi10.1111/1462-2920.13376-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://doi.org/10.1111/1462-2920.13376es_ES
dc.identifier.e-issn1462-2920-
dc.contributor.funderMinisterio de Ciencia, Tecnología e Innovación Productiva (Argentina)es_ES
dc.relation.csicSíes_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003033es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.languageiso639-1en-
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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