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Título: | Synthesis of oligodeoxynucleotides containing 6-N-([13C]methyl)adenine and 2-N-([13C]methyl)guanine |
Autor: | Hofmann, Mechtild; Acedo, Montse; Fagan, Patricia A.; Wemmer, David E.; Eritja Casadellà, Ramón CSIC ORCID ; Díaz, Antonio R. | Palabras clave: | Oligonucleotides NMR studies |
Fecha de publicación: | 21-jun-1997 | Editor: | Royal Society of Chemistry (UK) | Citación: | Journal of the Chemical Society, Perkin Transactions 1 12: 1825-1828 (1997) | Resumen: | Oligonucleotides containing 6-N-([l3C]methyl)adenine and 2-N-([13C]methyl)guanine have been prepared for NMR studies using the deprotection step to introduce the [13C]methylamine group. For this purpose, the use of 2′-deoxy-6-O-(pentafluorophenyl)inosine 1 and 2′-deoxy-2-fluoro-6-O-[2-(4-nitrophenyl)-ethyl]inosine 2 as precursors of the N-methylated nucleosides is described. Preliminary NMR characterization of the 13C-labelled oligonucleotides shows that the 13C chemical shift of the methyl group in N-methylguanine is sensitive to duplex formation, making it a useful local probe. | Versión del editor: | http://dx.doi.org/10.1039/A700366H | URI: | http://hdl.handle.net/10261/136409 | DOI: | 10.1039/A700366H | ISSN: | 0300922X |
Aparece en las colecciones: | (IQAC) Artículos |
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JCS Perkin Trans 1997C13.pdf | 164,13 kB | Adobe PDF | Visualizar/Abrir |
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