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Título: | Sinorhizobium meliloti low molecular weight phosphotyrosine phosphatase SMc02309 modifies activity of the UDP-glucose pyrophosphorylase ExoN involved in succinoglycan biosynthesis |
Autor: | Medeot, Daniela B.; Rivero, María Romina; Cendoya, Eugenia; Contreras-Moreira, Bruno CSIC ORCID ; Rossi, Fernando A.; Fischer, Sonia E.; Becker, Anke; Jofré, Edgardo | Fecha de publicación: | mar-2016 | Editor: | Society for General Microbiology | Citación: | Microbiology 162 (3): 552-563 (2016) | Resumen: | n Gram-negative bacteria, tyrosine phosphorylation has been shown to play a role in the control of exopolysaccharide (EPS) production. This study demonstrated that the chromosomal ORF SMc02309 from Sinorhizobium meliloti 2011 encodes a protein with significant sequence similarity to low molecular mass protein-tyrosine phosphatases (LMW-PTPs), such as the Escherichia coli Wzb. Unlike other well-characterized EPS biosynthesis gene clusters, which contain neighbouring LMW-PTPs and kinase, the S. meliloti succinoglycan (EPS I) gene cluster located on megaplasmid pSymB does not encode a phosphatase. Biochemical assays revealed that the SMc02309 protein hydrolyses p-nitrophenyl phosphate (p-NPP) with kinetic parameters similar to other bacterial LMW-PTPs. Furthermore, we show evidence that SMc02309 is not the LMW-PTP of the bacterial tyrosine-kinase (BY-kinase) ExoP. Nevertheless, ExoN, a UDP-glucose pyrophosphorylase involved in the first stages of EPS I biosynthesis, is phosphorylated at tyrosine residues and constitutes an endogenous substrate of the SMc02309 protein. Additionally, we show that the UDP-glucose pyrophosphorylase activity is modulated by SMc02309-mediated tyrosine dephosphorylation. Moreover, a mutation in the SMc02309 gene decreases EPS I production and delays nodulation on Medicago sativa roots. | Descripción: | 49 Pags.- 3 Tabls.- 5 Figs.- 2 Supp. Figs. The definitive version is available at: | Versión del editor: | http://dx.doi.org/10.1099/mic.0.000239 | URI: | http://hdl.handle.net/10261/130776 | DOI: | 10.1099/mic.0.000239 | ISSN: | 1350-0872 | E-ISSN: | 1465-2080 |
Aparece en las colecciones: | (EEAD) Artículos |
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ContrerasB_Microbiology _2016.pdf | 1,1 MB | Adobe PDF | Visualizar/Abrir |
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