English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/129766
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus or Perkinsus olseni challenge using an immune-enriched oligo-microarray

AuthorsMoreira, Rebeca ; Romero Jódar, Alejandro ; Milan, Massimo; Bargelloni, Luca; Novoa, Beatriz ; Figueras Huerta, Antonio
KeywordsManila clam
Hemocytes
Vibrio alginolyticus
Perkinsus olseni
Microarray
Immune response
Gene Ontology
Blast2GO
Issue Date2015
PublisherUniversidad de Santiago de Compostela
CitationInternational Symposium on Genetics in Aquaculture (2015)
AbstractThe Manila clam (Ruditapes philippinarum) is a cultured bivalve with worldwide commercial importance, and diseases in this species can cause high economic losses. For this reason, interest in the immune genes in this species has recently increased. The present work describes the construction of the first R. philippinarum microarray containing immune-related hemocyte sequences and the application of this microarray to study the gene transcription profiles of hemocytes from clams that were challenged with the bacteria Vibrio alginolyticus or the protistan parasite Perkinsus olseni through a time course. Our results highlight the importance of a fast response in bivalves and the effectiveness of their innate immune system. With regard to the V. alginolyticus challenge, genes related to signaling, transcription and apoptosis were typically expressed as early as 3 hours post-challenge (hpc), while characteristic immune genes appeared later at 8 hpc. This immune-triggering response could have affected a high number of processes that seemed to be activated 24 hpc to overcome the Vibrio challenge, including the expression of many cytoskeleton molecules, which is indicative of the active movement of hemocytes. In fact, functional studies showed an increment in apoptosis, necrosis or cell migration after the infection. Finally, 72 hpc, activity returned to normal levels. The results show that the key point to overcome the challenge seemed to be 8 hpc, when we detected immune functions that could lead to the destruction of the pathogen and the activation of a wide variety of processes related to homeostasis and defense. The analysis of the transcriptome profile after P. olseni infection revealed an early phase of infection (day 5) that was characterized by no mortality and by the increased expression of genes associated with pathogen recognition, production of nitrogen radicals and antimicrobial activity. This phase was followed by an intermediate stage (days 10 and 14), when the pathogen was most likely multiplying and infecting new areas of the body, and animals began to die. In this stage, many genes related to cell movement were over-expressed. Thirty days after infection metabolic pathway genes were the most affected. Apoptosis appears to be important during pathogenesis
DescriptionComunicación presentada en el XII International Symposium on Genetics in Aquaculture, Santiago de Compostela, 21-27 de junio de 2015
Publisher version (URL)http://isga2015.acuigen.es/isga-2015-Abstract-Book.pdf
URI10261/129766
Appears in Collections:(IIM) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
Gene_expression_profile.pdfPresentación3,13 MBAdobe PDFThumbnail
View/Open
Gene_expression_profile_abstract.pdfAbstract69,91 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.