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dc.contributor.authorMoreira, Rebecaes_ES
dc.contributor.authorRomero Jódar, Alejandroes_ES
dc.contributor.authorMilan, Massimoes_ES
dc.contributor.authorBargelloni, Lucaes_ES
dc.contributor.authorNovoa, Beatrizes_ES
dc.contributor.authorFigueras Huerta, Antonioes_ES
dc.date.issued2015-
dc.identifier.citationInternational Symposium on Genetics in Aquaculture (2015)es_ES
dc.identifier.uri10261/129766-
dc.descriptionComunicación presentada en el XII International Symposium on Genetics in Aquaculture, Santiago de Compostela, 21-27 de junio de 2015es_ES
dc.description.abstractThe Manila clam (Ruditapes philippinarum) is a cultured bivalve with worldwide commercial importance, and diseases in this species can cause high economic losses. For this reason, interest in the immune genes in this species has recently increased. The present work describes the construction of the first R. philippinarum microarray containing immune-related hemocyte sequences and the application of this microarray to study the gene transcription profiles of hemocytes from clams that were challenged with the bacteria Vibrio alginolyticus or the protistan parasite Perkinsus olseni through a time course. Our results highlight the importance of a fast response in bivalves and the effectiveness of their innate immune system. With regard to the V. alginolyticus challenge, genes related to signaling, transcription and apoptosis were typically expressed as early as 3 hours post-challenge (hpc), while characteristic immune genes appeared later at 8 hpc. This immune-triggering response could have affected a high number of processes that seemed to be activated 24 hpc to overcome the Vibrio challenge, including the expression of many cytoskeleton molecules, which is indicative of the active movement of hemocytes. In fact, functional studies showed an increment in apoptosis, necrosis or cell migration after the infection. Finally, 72 hpc, activity returned to normal levels. The results show that the key point to overcome the challenge seemed to be 8 hpc, when we detected immune functions that could lead to the destruction of the pathogen and the activation of a wide variety of processes related to homeostasis and defense. The analysis of the transcriptome profile after P. olseni infection revealed an early phase of infection (day 5) that was characterized by no mortality and by the increased expression of genes associated with pathogen recognition, production of nitrogen radicals and antimicrobial activity. This phase was followed by an intermediate stage (days 10 and 14), when the pathogen was most likely multiplying and infecting new areas of the body, and animals began to die. In this stage, many genes related to cell movement were over-expressed. Thirty days after infection metabolic pathway genes were the most affected. Apoptosis appears to be important during pathogenesises_ES
dc.language.isoenges_ES
dc.publisherUniversidad de Santiago de Compostelaes_ES
dc.rightsopenAccesses_ES
dc.subjectManila clames_ES
dc.subjectHemocyteses_ES
dc.subjectVibrio alginolyticuses_ES
dc.subjectPerkinsus olsenies_ES
dc.subjectMicroarrayes_ES
dc.subjectImmune responsees_ES
dc.subjectGene Ontologyes_ES
dc.subjectBlast2GOes_ES
dc.titleGene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus or Perkinsus olseni challenge using an immune-enriched oligo-microarrayes_ES
dc.typecomunicación de congresoes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://isga2015.acuigen.es/isga-2015-Abstract-Book.pdfes_ES
dc.relation.csices_ES
Appears in Collections:(IIM) Comunicaciones congresos
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