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Título: | Flavin-mediated reduction of ferric leghemoglobin from soybean nodules |
Autor: | Becana Ausejo, Manuel CSIC ORCID ; Salin, Marvin L.; Ji, Lin; Klucas, R. V. | Palabras clave: | Flavin Glycine (nitrogen fixation) Leghemoglobin Nitrogen fixation Oxygen (activated) Pyridine nucleotides Symbiosis (legume-Rhizobium) |
Fecha de publicación: | mar-1991 | Editor: | Springer Nature | Citación: | Planta 183 (4): 575-583 (1991) | Resumen: | The reduction of ferric leghemoglobin (Lb3+) from soybean (Glycine max (L.) Merr.) nodules by riboflavin, FMN and FAD in the presence of NAD(P)H was studied in vitro. The system NAD(P)H + flavin reduced Lb3+ to oxyferrous (Lb2+ · O2) or deoxyferrous (Lb2+) leghemoglobin in aerobic or anaerobic conditions, respectively. In the absence of O2 the reaction was faster and more effective (i.e. less NAD(P)H oxidized per mole Lb3+ reduced) than in the presence of O2; this phenomenon was probably because O2 competes with Lb3+ for reductant, thus generating activated O2 species. The flavin-mediated reduction of Lb3+ did not entail production of superoxide or peroxide, indicating that NAD(P)H-reduced flavins were able to reduce Lb3+ directly. The NAD(P)H + flavin system also reduced the complexes Lb3+ · nicotinate and Lb3+ · acetate to Lb2+ · O2, Lb2+ or Lb2+ · nicotinate, depending on the concentrations of ligands and of O2. In the presence of 200 μM nitrite most Lb remained as Lb3+ in aerobic conditions but the nitrosyl complex (Lb2+ · NO) was generated in anaerobic conditions. The above-mentioned characteristics of the NAD(P)H + flavin system, coupled with its effectiveness in reducing Lb3+ at physiological levels of NAD(P)H and flavins in soybean nodules, indicate that this mechanism may be especially important for reducing Lb3+ in vivo. | Descripción: | 22 Pags. The definitive version, with Figs., is available at: http://link.springer.com/journal/425 | Versión del editor: | http://dx.doi.org/10.1007/BF00194279 | URI: | http://hdl.handle.net/10261/109134 | DOI: | 10.1007/BF00194279 | ISSN: | 0032-0935 | E-ISSN: | 1432-2048 |
Aparece en las colecciones: | (EEAD) Artículos |
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