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Título: | Relation between serologic response and pathologic findings in sheep with naturally acquired paratuberculosis |
Autor: | Pérez Pérez, Valentín CSIC ORCID ; Tellechea, J.; Badiola Díez, Juan José; Gutiérrez, M.; García Marín, Juan Francisco CSIC | Palabras clave: | Infection Cattle Diagnosis Johnes Disease Mycobacterium-Paratuberculosis Para-Tuberculosis Agar-Gel Immunodiffusion Complement-Fixation Test |
Fecha de publicación: | 1997 | Editor: | American Veterinary Medical Association | Citación: | American Journal of Veterinary Research 58: 799- 803 (1997) | Resumen: | Objective-To investigate the relation between lesions and serologic response in sheep with naturally acquired paratuberculosis and to evaluate the efficacy of agar gel immunodiffusion (AGID) and ELISA as diagnostic tests. Animals-134 adult sheep, culled by the owner from flocks with history of severe chronic weight loss. Procedure-Before euthanasia, blood samples were collected and serum was obtained. AGID and ELISA were performed, using a protoplasmic antigen of Mycobacterium paratuberculosis. Sensitivity and specificity of these techniques were evaluated, using histopathologic findings as a reference. Histologic studies were carried out focusing on specimens containing intestinal organized lymphoid tissue. Results-Of the sheep examined, 46.3% had lesions that were classified in 5 categories, according to their location, extension, cellular types, and presence of acid-fast bacilli. Regarding serologic test results, 37.1% of sheep with lesions had a positive AGID result, and all sheep without lesions had negative results. However, ELISA positivity was slightly greater-48.4% of sheep with lesions; however, by use of ELISA, 11.1% of sheep without lesions had positive results. Conclusion-A close relation between pathologic findings and serologic response was determined, especially between presence of acid-fast bacilli (potentially heavily shedding sheep) in the intestinal lesions and positivity to serologic tests, especially AGID. Clinical Relevance-On the basis of these results, together with its high specificity, reliability, and the difficulty of fecal specimen culture in sheep, AGID is proposed as a useful tool for seeking evidence of paratuberculosis in live sheep, and therefore, is suggested as the basis for control programs in areas in which the disease is a serious threat to sheep production. | Descripción: | 5 páginas. | Versión del editor: | http://dx.doi.org/https://www.avma.org/Pages/home.aspx | URI: | http://hdl.handle.net/10261/103950 | DOI: | https://www.avma.org/Pages/home.aspx | ISSN: | 0002-9645 |
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