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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Pérez-Lago, Laura | - |
dc.contributor.author | Salas, Margarita | - |
dc.contributor.author | Camacho, Ana | - |
dc.date.accessioned | 2008-10-16T13:58:11Z | - |
dc.date.available | 2008-10-16T13:58:11Z | - |
dc.date.issued | 2005-01-07 | - |
dc.identifier.citation | Nucleic Acids Research 2005 33(1):126-134 | en_US |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10261/7829 | - |
dc.description.abstract | Bacteriophage phi 29 protein p4 is essential for the regulation of the switch from early to late phage transcription. The protein binds to two regions of the phage genome located between the regulated promoters. Each region contains two inverted repeats separated by 1 bp. We used circular permutation assays to study the topology of the DNA upon binding of the protein and found that p4 induced the same extent of bending independent of the topology of the binding region. In addition, the results revealed that the p4-induced bending is not dependent on the affinity to the binding site but is intrinsic to p4 binding. Independent binding sites were identified through the characterization of the minimal sequence required for p4 binding. The protein has different affinity for each of its binding sites, with those overlapping the A2c and A2b promoter cores (sites 1 and 3), having the highest affinity. The functionality of the p4 binding sites and the contribution of p4-mediated promoter restructuring in transcription regulation is discussed | en_US |
dc.description.sponsorship | This work was funded by grant 08.2/0026.1/2003 of the Comunidad de Madrid to A.C., grants 2R01 GM27242-24 from the National Institutes of Health and BMC 2002-03818 from the Spanish Ministry of Science and Technology to M.S. and by an Institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular ‘Severo Ochoa’. L.P.-L. is a holder of a pre-doctoral fellowship from Comunidad Autónoma de Madrid. The ‘Ramón y Cajal’ program of the Spanish Ministry of Science and Technology supported A.C. Funding to pay the Open Access publication charges for this article was provided by the Comunidad de Madrid. | en_US |
dc.format.extent | 856190 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | openAccess | en_US |
dc.subject | Bacteriophage phi 29 | en_US |
dc.title | A precise DNA bend angle is essential for the function of the phage 29 transcriptional regulator | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1093/nar/gki146 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/gki146 | en_US |
dc.identifier.e-issn | 1362-4962 | - |
dc.contributor.funder | Comunidad de Madrid | - |
dc.contributor.funder | National Institutes of Health (US) | - |
dc.contributor.funder | Ministerio de Ciencia y Tecnología (España) | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100000002 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100006280 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.identifier.pmid | 15642698 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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