Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/73990
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Ruiz-Ferrer, Virginia | - |
dc.contributor.author | Goytia, Elisa | - |
dc.contributor.author | Martínez-García, Belén | - |
dc.contributor.author | López-Abella, Dionisio | - |
dc.contributor.author | López-Moya Gómez, Juan José | - |
dc.date.accessioned | 2013-04-10T08:50:32Z | - |
dc.date.available | 2013-04-10T08:50:32Z | - |
dc.date.issued | 2004-01-01 | - |
dc.identifier.citation | Journal of General Virology, 85 (1) : 241-249 | es_ES |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | http://hdl.handle.net/10261/73990 | - |
dc.description | 9 páginas, 3 figuras, 1 tabla -- PAGS NROS. 241-249 | es_ES |
dc.description.abstract | Tobacco etch potyvirus (TEV) is transmitted by aphids in a non-persistent manner with the assistance of a virus-encoded protein known as helper component (HC-Pro). To produce a biologically active form of recombinant TEV HC-Pro protein, heterologous expression in the methylotrophic yeast Pichia pastoris was used. A cDNA encoding the TEV HC-Pro region, fused to a Saccharomyces cerevisiae α-mating factor secretory peptide coding region, was inserted into the P. pastoris genome using a modified version of the pPIC9 vector. The expressed TEV HC-Pro protein was obtained directly from culture medium of recombinant yeast colonies; it was able to interact with TEV particles in a protein overlay binding assay, and also to assist aphid transmission of purified TEV particles to plants using the aphid Myzus persicae as vector. Our results indicate that P. pastoris provides a rapid and low-cost heterologous expression system that can be used to obtain biologically active potyvirus HC-Pro protein for in vitro transmission assays | es_ES |
dc.description.sponsorship | This work was supported by grants BIO2000-0914, BIO 2000-1605-C02-02 and AGL2001-2141 from CICYT (Spain), and 07M-0123-2000 and 07M-0072-2002 from Comunidad de Madrid (Spain). V. R.-F. and E. G. are recipients of PNFPI fellowships from MCyT (Spain), and B. M.-G. is the recipient of a post-doctoral contract from the Comunidad de Madrid (Spain). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Society for General Microbiology | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | Expresión of functionally active helper component protein of Tobacco etch potyvirus in the yeast Pichia pastoris | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1099/vir.0.19557-0 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1099/vir.0.19557-0 | es_ES |
dc.identifier.e-issn | 1465-2099 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
8
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
6
checked on 26-feb-2024
Page view(s)
492
checked on 30-abr-2024
Download(s)
171
checked on 30-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.