Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/51067
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Processing and immobilization of enzyme Ribonuclease A through laser irradiation |
Autor: | Popescu, C.; Roqueta, Jaume CSIC; Pérez del Pino, Ángel CSIC ORCID; Gyorgy, Eniko CSIC ORCID | Fecha de publicación: | 2011 | Editor: | Cambridge University Press | Citación: | Journal of Materials Research 26: 815-821 (2011) | Resumen: | We report the processing and immobilization of enzyme Ribonuclease A (RNase A) onto SiO2 glass collectors using the matrix assisted pulsed laser evaporation (MAPLE) technique. The experiments were performed inside a stainless steel irradiation chamber. A pulsed UV KrF* (λ = 248 nm, τFWHM ≈ 25 ns, ν = 10 Hz) excimer laser source was used for the irradiations. The laser fluence was varied in the range 0.4-0.7 J/cm 2. The morphology of the obtained films was investigated by atomic force microscopy (AFM) and their structure and composition by Fourier transform infrared spectroscopy (FTIR). The FTIR spectra of the films obtained from composite MAPLE targets consisting of 1% (w/v) RNase A in Hepes-KOH 10 mM pH 7.5 buffer exhibit the same bands as the spectrum of the initial, nonirradiated material. The enzymatic activity of the obtained structures was analyzed using synthetic substrate polycytidylic acid (poly(C)). The poly (C) cleavage by the immobilized enzyme and the products of formation were analyzed by means of reverse phase high performance liquid chromatography (HPLC). © 2011 Materials Research Society. | URI: | http://hdl.handle.net/10261/51067 | DOI: | 10.1557/jmr.2010.55 | Identificadores: | doi: 10.1557/jmr.2010.55 issn: 0884-2914 |
Aparece en las colecciones: | (ICMAB) Artículos (CIN2) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Processing and immobilization of enzyme Ribonuclease.pdf | 277,39 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
12
checked on 03-may-2024
WEB OF SCIENCETM
Citations
12
checked on 21-feb-2024
Page view(s)
282
checked on 03-may-2024
Download(s)
316
checked on 03-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.