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Título: | Effects of internal deletions on the priming activity of the phage ø29 terminal protein |
Autor: | Zaballos, Ángel; Lázaro, José M. CSIC; Méndez Cormán, Enrique CSIC; Mellado, Rafael P.; Salas, Margarita CSIC ORCID | Palabras clave: | Recombinant DNA Phage lambda PL promoter Protein p3-dAMP complex |
Fecha de publicación: | 30-nov-1989 | Editor: | Elsevier | Citación: | Gene 83(2): 33-40 (1989) | Resumen: | A series of internal deletions of gene 3, coding for the phage φ29 DNA terminal protein, have been constructed and characterized. In addition, a substitution mutant in the sequence corresponding to amino acids (aa) 49–51 was obtained. The priming activity of the substitution mutant protein, m the formation of the protein p3-dAMP initiation complex, was drastically reduced suggesting that some of the aa present at position 49–51 are essential for p3 function. Deletions of 8 to 33 aa, from aa residue 48 towards the N terminus of the substitution mutant, further decreased the priming activity of the protein. The activity of deletion mutants lacking 15 or 21 aa from residue 57 towards the C terminus, and also containing a point mutation at position 56, was greatly reduced, and no activity was seen when 24 aa were lacking. | Versión del editor: | http://dx.doi.org/10.1016/0378-1119(89)90104-2 | URI: | http://hdl.handle.net/10261/39256 | DOI: | 10.1016/0378-1119(89)90104-2 | ISSN: | 0378-1119 |
Aparece en las colecciones: | (CBM) Artículos |
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