Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/353153COMPARTIR / EXPORTAR:
 SHARE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Supplementary Information: Neuronal progenitors of the dentate gyrus express the SARS-CoV-2 cell receptor during migration in the developing human hippocampus |
Autor: | Hernández-López, José Manuel; Hernández-Medina, Cristina; Medina-Corvalan, Cristina; Ródenas, Mónica CSIC; Almagro-García, Francisca CSIC; Pérez-García, Claudia; Echevarría, Diego CSIC ORCID; Carratalá-Marco, Francisco; Geijo-Barrientos, Emilio CSIC ORCID; Martínez, Salvador CSIC ORCID | Fecha de publicación: | 2023 | Editor: | Springer Nature | Citación: | Hernández-López, José Manuel; Hernández-Medina, Cristina; Medina-Corvalan, Cristina; Ródenas, Mónica; Almagro-García, Francisca; Pérez-García, Claudia; Echevarría, Diego; Carratalá-Marco, Francisco; Geijo-Barrientos, Emilio; Martínez, Salvador; 2023; Supplementary Information: Neuronal progenitors of the dentate gyrus express the SARS-CoV-2 cell receptor during migration in the developing human hippocampus [Dataset]; Springer Nature; https://doi.org/10.1007/s00018-023-04787-8 | Descripción: | Supplementary Figure 1: Control of immunolabelling and ACE2 expression in adult hippocampus. A-C) Pictures of the hippocampus fimbrial angle in control processed sections without primary ant-ACE2 antibodies: A) rabbit polyclonal anti-ACE2 (Abcam Cat# ab15348). Control sections processed with rabbit polyclonal anti-ACE2 (Sigma-Aldrich Cat#HPA000288) and mouse monoclonal anti-ACE2 (R&D Systems Cat# MAB933) were similar to this one (data not shown). B) rat monoclonal anti-GFAP (Millipore Cat# 345860-100UG). Control sections processed with goat polyclonal anti-Doublecortin (Santa Cruz Biotechnology Cat# sc-8066) were similar to this one (data not shown); C) rabbit polyclonal anti-TBR2 (Abcam Cat# ab23345). D-G) ACE2 expression has been detected in paraffine section of adult hippocampus, demonstrating specific expression of ACE2 (rabbit ati-ACE2 from Abcam) in astroglia in D, E and F (arrows), and pericytes in G (arrow). While ACE2-expressing cells are identified by brown-colored neurons were negative as we can observe in D by the white color (arrowhead). H) Double immunohistochemistry showing GFAP expression (brown) and ACE2 expression (black dots; arrows) in astroglial cells. I) Control section processed with GFAP and without ACE2 antibodies, showing the GFAP derived immunoreactivity and absence of ACE2 immunostaining. Scale bar: D-I) 50 µm. Supplementary Figure 2 is not the revised version and has to be changed by the uploaded one, which contains the revised lettering: Specificity of ACE2 expression in human lung, kidney and developing brain. A-C) The three antibodies show ACE2-specific immunoreaction in human lung in alveolar endothelial type II cells (arrows) and capillary endothelium (arrowheads). D-F) The three antibodies show ACE2-specific immunoreaction in human renal proximal tubule cells (arrows). G) Low power picture of a brain section processed in parallel by immunohistochemistry without primary ACE2 Sigma-Aldrich antibody and counterstained with Cresyl violet. H) High power picture of a brain section processed in parallel by immunohistochemistry without primary ACE2 R&D antibody and counterstained with Cresyl violet. Control sections without primary antibodies do not showed immunostaining. I-N) The three antibodies show ACE2-specific immunoreaction in DG progenitors and migrating cells in DGMS. Scale bar: A, B, C) 50 µm; D, E, F) 25 µm; G, I, K, M) 100 µm; J, L, N) 25 µm. DGMS: Dentate Gyrus Migratory Stream; IZ: Intermediate Zone; SVZ: Subventricular zone: VE: Ventricular Epithelium. | URI: | http://hdl.handle.net/10261/353153 | DOI: | 10.1007/s00018-023-04787-8 | Referencias: | Hernández-López, José Manuel; Hernández-Medina, Cristina; Medina-Corvalan, Cristina; Ródenas, Mónica; Almagro-García, Francisca; Pérez-García, Claudia; Echevarría, Diego; Carratalá-Marco, Francisco; Geijo-Barrientos, Emilio; Martínez, Salvador. Neuronal progenitors of the dentate gyrus express the SARS-CoV-2 cell receptor during migration in the developing human hippocampus. https://doi.org/10.1007/s00018-023-04787-8. http://hdl.handle.net/10261/353151 |
| Aparece en las colecciones: | (IN) Conjuntos de datos (PTI Salud Global) Colección Especial COVID-19 |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| 18_2023_4787_MOESM1_ESM.pdf | 9,24 MB | Adobe PDF | Visualizar/Abrir | |
| 18_2023_4787_MOESM2_ESM.pdf | 21,36 MB | Adobe PDF | Visualizar/Abrir | |
| README.txt | 3,08 kB | Text | Visualizar/Abrir |
CORE Recommender
Page view(s)
13
checked on 28-may-2024
Download(s)
4
checked on 28-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.