Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/351878
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Factor H-related protein 1 promotes complement-mediated opsonization of Pseudomonas aeruginosa |
Autor: | González-Alsina, Álex; Martín Merinero, Héctor CSIC ORCID; Mateu-Borrás, Margalida; Verd, María; Doménech-Sánchez, Antonio CSIC ORCID; Goldberg, Joanna B.; Rodríguez de Córdoba, Santiago ; Albertí, Sebastián | Palabras clave: | FHR-1 Factor H Complement system P. aeruginosa OprG |
Fecha de publicación: | 6-mar-2024 | Editor: | Frontiers Media | Citación: | Frontiers in Cellular and Infection Microbiology 14: 1328185 (2024) | Resumen: | Pseudomonas aeruginosa is an important human opportunistic pathogen responsible for a wide range of infections. The complement system is the main early host defense mechanism to control these infections. P. aeruginosa counteracts complement attack by binding Factor H (FH), a complement regulator that inactivates C3b, preventing the formation of the C3-convertase and complement amplification on the bacterial surface. Factor H-related proteins (FHRs) are a group of plasma proteins evolutionarily related to FH that have been postulated to interfere in this bacterial mechanism of resisting complement. Here, we show that FHR-1 binds to P. aeruginosa via the outer membrane protein OprG in a lipopolysaccharide (LPS) O antigen-dependent manner. Binding assays with purified components or with FHR-1-deficient serum supplemented with FHR-1 show that FHR-1 competes with FH for binding to P. aeruginosa. Blockage of FH binding to C3b deposited on the bacteria reduces FH-mediated cofactor activity of C3b degradation, increasing the opsonization of the bacteria and the formation of the potent chemoattractant C5a. Overall, our findings indicate that FHR-1 is a host factor that promotes complement activation, facilitating clearance of P. aeruginosa by opsonophagocytosis. | Descripción: | 10 p.-6 fig. | Versión del editor: | https://doi.org/10.3389/fcimb.2024.1328185 | URI: | http://hdl.handle.net/10261/351878 | DOI: | 10.3389/fcimb.2024.1328185 | E-ISSN: | 2235-2988 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
fcimb_gonzález-alsina_2024.pdf | Artículo principal | 4,68 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Este item está licenciado bajo una Licencia Creative Commons