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Título: | The Production of Recombinant African Swine Fever Virus Lv17/WB/Rie1 Strains and Their In Vitro and In Vivo Characterizations |
Autor: | Petrini, Stefano; Righi, Cecilia; Mészáros, Itzván; D'Errico, Federica; Tamás, Vivien; Pela, Michela; Olasz, Ferenc; Gallardo, Carmina; Fernández-Pinero, Jovita; Göltl, Eszter; Magyar, Tibor; Feliziani, Francesco; Zádori, Zoltán | Palabras clave: | ASFV African swine fever Homologous recombination Illegitimate recombination Live-attenuated vaccine Pigs |
Fecha de publicación: | 17-dic-2023 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Vaccines 11(12): e1860 (2023) | Resumen: | Lv17/WB/Rie1-Δ24 was produced via illegitimate recombination mediated by low-dilution serial passage in the Cos7 cell line and isolated on PAM cell culture. The virus contains a huge ~26.4 Kb deletion in the left end of its genome. Lv17/WB/Rie1-ΔCD-ΔGL was generated via homologous recombination, crossing two ASFV strains (Lv17/WB/Rie1-ΔCD and Lv17/WB/Rie1-ΔGL containing eGFP and mCherry markers) during PAM co-infection. The presence of unique parental markers in the Lv17/WB/Rie1-ΔCD-ΔGL genome indicates at least two recombination events during the crossing, suggesting that homologous recombination is a relatively frequent event in the ASFV genome during replication in PAM. Pigs infected with Lv17/WB/Rie1-Δ24 and Lv17/WB/Rie1/ΔCD-ΔGL strains have shown mild clinical signs despite that ASFV could not be detected in their sera until a challenge infection with the Armenia/07 ASFV strain. The two viruses were not able to induce protective immunity in pigs against a virulent Armenia/07 challenge. | Descripción: | 18 Pág. | Versión del editor: | https://doi.org/10.3390/vaccines11121860 | URI: | http://hdl.handle.net/10261/348157 | DOI: | 10.3390/vaccines11121860 | ISSN: | 2076-393X |
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The_Production_of_Recombinant.pdf | artículo | 2,29 MB | Adobe PDF | Visualizar/Abrir |
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