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Título: | Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations |
Autor: | Horváth, Vivien CSIC ORCID; Guirao-Rico, Sara CSIC ORCID; Salces-Ortiz, Judit CSIC ORCID; Rech, Gabriel E. CSIC ORCID; Green, Llewellyn; Aprea, Eugenio; Rodeghiero, Mirco; Anfora, Gianfranco; González Pérez, Josefa CSIC ORCID | Palabras clave: | Cuticular hydrocarbons Water content Respiration rate Post-transcriptional regulation tRFs Insect physiology |
Fecha de publicación: | 16-feb-2023 | Editor: | Springer Nature | Citación: | BMC Biology 21: 35 (2023) | Resumen: | [Background] Climate change is one of the main factors shaping the distribution and biodiversity of organisms, among others by greatly altering water availability, thus exposing species and ecosystems to harsh desiccation conditions. However, most of the studies so far have focused on the effects of increased temperature. Integrating transcriptomics and physiology is key to advancing our knowledge on how species cope with desiccation stress, and these studies are still best accomplished in model organisms. [Results] Here, we characterized the natural variation of European D. melanogaster populations across climate zones and found that strains from arid regions were similar or more tolerant to desiccation compared with strains from temperate regions. Tolerant and sensitive strains differed not only in their transcriptomic response to stress but also in their basal expression levels. We further showed that gene expression changes in tolerant strains correlated with their physiological response to desiccation stress and with their cuticular hydrocarbon composition, and functionally validated three of the candidate genes identified. Transposable elements, which are known to influence stress response across organisms, were not found to be enriched nearby differentially expressed genes. Finally, we identified several tRNA-derived small RNA fragments that differentially targeted genes in response to desiccation stress. [Conclusions] Overall, our results showed that basal gene expression differences across individuals should be analyzed if we are to understand the genetic basis of differential stress survival. Moreover, tRNA-derived small RNA fragments appear to be relevant across stress responses and allow for the identification of stress-response genes not detected at the transcriptional level. |
Descripción: | © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | Versión del editor: | https://doi.org/10.1186/s12915-023-01530-4 | URI: | http://hdl.handle.net/10261/348061 | DOI: | 10.1186/s12915-023-01530-4 | ISSN: | 1741-7007 |
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Gene_BMC-Biology_2023_OA.pdf | 2,64 MB | Adobe PDF | Visualizar/Abrir |
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