Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/347115
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | In vitro modeling of polyclonal infection dynamics within the human airways by Haemophilus influenzae differential fluorescent labeling |
Autor: | Rapún-Araiz, Beatriz CSIC; Sorzabal-Bellido, Ioritz; Asensio-López, Javier; Lázaro-Díez, María CSIC ORCID; Ariz, Mikel; Sobejano de la Merced, Carlos; Euba, Begoña CSIC ORCID ; Fernández-Calvet, Ariadna CSIC ORCID; Cortés-Domínguez, Ivan; Burgui, Saioa CSIC ORCID; Toledo-Arana, Alejandro CSIC ORCID ; Ortiz-de-Solórzano, Carlos; Garmendia, Juncal CSIC ORCID | Palabras clave: | Haemophilus influenzae Fluorescent labeling Mixed biofilms Mixed epithelial infection Plasmid toolbox Polyclonality assessment Selective antibiotic efficacy |
Fecha de publicación: | 12-dic-2023 | Editor: | American Society for Microbiology | Citación: | Microbiology Spectrum 11(6): e00993-23 (2023) | Resumen: | Genomic diversity of nontypeable H. influenzae strains confers phenotypic heterogeneity. Multiple strains of H. influenzae can be simultaneously isolated from clinical specimens, but we lack detailed information about polyclonal infection dynamics by this pathogen. A long-term barrier to our understanding of this host-pathogen interplay is the lack of genetic tools for strain engineering and differential labeling. Here, we present a novel plasmid toolkit named pTBH (toolbox for Haemophilus), with standardized modules for fluorescent or bioluminescent labeling, adapted to H. influenzae requirements but designed to be versatile so it can be utilized in other bacterial species. We present detailed experimental and quantitative image analysis methods, together with proof-of-principle examples, and show the ample possibilities of 3D microscopy, combined with quantitative image analysis, to model H. influenzae polyclonal infection lifestyles and unravel the co-habitation and co-infection dynamics of this respiratory pathogen. | Versión del editor: | https://doi.org/10.1128/spectrum.00993-23 | URI: | http://hdl.handle.net/10261/347115 | DOI: | 10.1128/spectrum.00993-23 | E-ISSN: | 2165-0497 |
Aparece en las colecciones: | (IDAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | Existing users please Login |
---|---|---|---|---|
rapún-araiz-et-al-2023-in-vitro-modeling-of-polyclonal-infection-dynamics-within-the-human-airways-by-haemophilus.pdf | 4,33 MB | Adobe PDF | Embargoed until 12 de junio de 2024 Petición de una copia |
CORE Recommender
Este item está licenciado bajo una Licencia Creative Commons