Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/344868
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Development of a real-time PCR assay for detection and quantification of Enteromyxum scophthalmi parasites in turbot intestinal samples |
Autor: | Piazzon de Haro, María Carla CSIC ORCID ; Mallo, Natalia; Martín, Iris; Fernández Casal, Jacobo; Sanmartín, Manuel L.; Lamas, Jesús; Leiro, José M. | Palabras clave: | Turbot Enteromyxum scophthalmi Myxozoan parasite Quantitative PCR Diagnosis |
Fecha de publicación: | 15-sep-2012 | Editor: | Elsevier | Citación: | Aquaculture | Resumen: | The myxozoan parasite Enteromyxum scophthalmi causes severe enteritis in cultured turbot Scophthalmus maximus, thus generating important economic losses. At present, there are no prevention or control measures for the disease, and many aspects of the life cycle and transmission of the parasite are not yet known. In this study, a highly sensitive, reproducible and rapid quantitative (real time) polymerase chain reaction (qPCR) assay was developed to detect E. scophthalmi DNA. The qPCR assay targets the 28S rRNA gene of the parasite, which has a high identity (94%) with the myxosporidian Enteromyxum leei rRNA gene. The qPCR assay was able to detect up to 13 DNA copies, corresponding to 0.55 fg, estimating that genomic DNA has around 1450 copies of 28S rRNA gene per parasite nucleus. The mean intra- and inter-assay coefficients of variation were below 5% and no detectable amplification was observed with DNA from non-infected turbot. The assay was validated with a histological identification of intestinal content samples from experimentally infected turbot and a good correlation between both methods was observed. The results demonstrate that the qPCR assay can be applied in the diagnosis of turbot enteromyxosis and to determine the relative abundance of E. scophthalmi in turbot intestinal contents in health monitoring studies. | Versión del editor: | http://dx.doi.org/10.1016/j.aquaculture.2012.09.008 | URI: | http://hdl.handle.net/10261/344868 | DOI: | 10.1016/j.aquaculture.2012.09.008 |
Aparece en las colecciones: | (IATS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | Artículo de acceso restringido por la editorial | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
7
checked on 11-may-2024
WEB OF SCIENCETM
Citations
7
checked on 22-feb-2024
Page view(s)
11
checked on 16-may-2024
Download(s)
2
checked on 16-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.