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Título: | Bacillus subtilis RarA modulates replication restart |
Autor: | Carrasco, Begoña; Seco, Elena M; López-Sanz, María; Alonso, Juan Carlos CSIC ORCID ; Ayora, Silvia CSIC ORCID | Palabras clave: | replicación de DNA Estabilidad genómica Bacillus subtilis |
Tesauro UNESCO: | Bioquímica Bacteria Biología Molecular |
Fecha de publicación: | 2018 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research | Resumen: | The ubiquitous RarA/Mgs1/WRNIP protein plays a crucial, but poorly understood role in genome maintenance. We show that Bacillus subtilis RarA, in the apo form, preferentially binds single-stranded (ss) over double-stranded (ds) DNA. SsbA bound to ss-DNA loads RarA, and for such recruitment the amphipathic C-terminal domain of SsbA is required. RarA is a DNA-dependent ATPase strongly stimulated by ssDNA–dsDNA junctions and SsbA, or by dsDNA ends. RarA, which may interact with PriA, does not stimulate PriA DNA unwinding. In a reconstituted PriA-dependent DNA replication system, RarA inhibited initiation, but not chain elongation. The RarA effect was not observed in the absence of SsbA, or when the host-encoded preprimosome and the DNA helicase are replaced by proteins from the SPP1 phage with similar function. We propose that RarA assembles at blocked forks tomaintain genome integrity. Through its interaction with SsbA and with a preprimosomal component, RarAmight impede the assembly of the replicative helicase, to prevent that recombination intermediates contribute to pathological DNA replication restart. | Versión del editor: | https://academic.oup.com/nar/article/46/14/7206/5045643?login=false | URI: | http://hdl.handle.net/10261/343175 | DOI: | 10.1093/nar/gky541 |
Aparece en las colecciones: | (CNB) Artículos |
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RarA_NAR_2018.pdf | Artículo publicado | 7,4 MB | Adobe PDF | Visualizar/Abrir |
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