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Título: | Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (NPPASE), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants |
Autor: | Muñoz Pérez, Francisco José CSIC ORCID ; Rodríguez López, Milagros; Baroja-Fernández, Edurne CSIC ORCID CVN ; Pozueta Romero, Javier CSIC ORCID ; Mitsui, Toshiaki; Nanjo, Yohei | Fecha de publicación: | 1-jun-2005 | Citación: | EP 1535998 (A1) | Resumen: | NPPase is an enzyme that catalyses the hydrolysis of a wide range of small molecules with phosphodiester and phosphosulphate bonds, in particular ADPG (adenosine diphosphate glucose) and APS (adenosine 5'-phosphosulphate). The enzyme obtained from plant extracts is used in assay devices for determining levels of nucleoside diphosphate sugars, based either on the sugar-1-phosphate released, or on the nucleoside monophosphate, both of which are products formed by the reaction catalysed by NPPase, as well as the detection of sulphonucleotides such as 3'-phosphoadenosine 5'-phosphosulphate (PAPS) and APS. The amino acid sequence of the enzyme is also described, as well as the nucleotide sequence of a complete cDNA and another incomplete cDNA. Finally, it describes the production of transgenic plants that overexpress NPPase and that have a high content of sugars, low content of starch and cell-wall polysaccharides and high resistance to high concentrations of salts and high temperature. | Descripción: | Fecha de presentación internacional: 15.07.2003. - Titulares: Consejo Superior de Investigaciones Científicas (CSIC). - Universidad Pública de Navarra. - Niigata University. | URI: | http://hdl.handle.net/10261/34240 |
Aparece en las colecciones: | (IDAB) Patentes |
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EP1535998A1.pdf | 1,44 MB | Adobe PDF | Visualizar/Abrir |
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