Supplementary Material for Low genetic diversity of the only clade of the tick Rhipicephalus microplus in the neotropics

Abstract

Figure S1: Asian–Neotropical Bayesian tree inferred with partial sequences of partial COI mtDNA (COI) using the GTR+G+I model of nucleotide substitution. Sequence data generated in the present study are highlighted in bold; Figure S2: Asian–Neotropical Bayesian tree inferred with partial sequences of partial 16S rDNA (16S) using the GTR+G model of nucleotide substitution. Sequence data generated in the present study are highlighted in bold; Figure S3: Asian–Neotropical Bayesian tree inferred with partial sequences of partial ITS2 using the GTR+G model of nucleotide substitution. Sequence data generated in the present study are highlighted in bold; Figure S4: Asian–Neotropical Bayesian tree inferred with concatenated partial sequences of the mitochondrial genes, COI and 16S rDNA using the GTR+G model of nucleotide substitution. Tables S1–S3: An excel-type spreadsheet including all the samples and the maximum likelihood reconstruction of the phylogenies based on COI, 16S, or ITS2 (in separate sheets). Each sheet includes the name as in the figures (phylogenetic trees) as well as the precedence (country), number of GenBank or BOLD accession number and reference (in case it was not from our own collections): Tables S4–S7: An excel-type spreadsheet including the similarities among samples based on COI, 16S, or ITS2 (in separate sheets). A fourth sheet includes the distance in km among geo-referenced samples (in a straight line). The fifth sheet includes the percent of environmental similarity among the ecological regions outlined for the phylogeographic study. All the data about similarities are in the range 0–100; Table S8: Alignment size (bp), number of segregating sites, number of haplotypes, haplotype diversity (Hd), nucleotide diversity (pi), and number of sequences of R. microplus.