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| Título: | Metadata for RNAseq project analysing differential expression in Culex pipiens mosquitoes infected by two avian Plasmodium species |
Autor: | Garrigós, Marta; Ylla, Guillem CSIC ORCID; Martínez de la Puente, Josué CSIC ORCID; Figuerola, Jordi CSIC ORCID ; Ruiz-López, María José CSIC ORCID | Palabras clave: | Transcriptomes Avian malaria Culex Gene expresion |
Fecha de publicación: | 12-dic-2023 | Editor: | DIGITAL.CSIC | Citación: | Garrigós, Marta; Ylla, Guillem; Martínez de la Puente, Josué; Figuerola, Jordi; Ruiz-López, María José; 2023; Metadata for RNAseq project analysing differential expression in Culex pipiens mosquitoes infected by two avian Plasmodium species [Dataset]; DIGITAL.CSIC; https://doi.org/10.20350/digitalCSIC/15708 | Resumen: | Metadata describing the experimental conditions of an RNA seq experiment that aims to analyse the differential gene expression of Culex pipiens when exposed to either Plasmodium relictum or Plasmodium cathemerium. The data includes the information of the corresponding project published at the European Nucleotide Archive (https://www.ebi.ac.uk/ena/browser/view/PRJEB41609), sample IDs and the links to the generated fasta files. | Descripción: | [Description of methods used for collection/generation of data] We collected birds infected by Plasmodium cathemerium and Plasmodium relictum and uninfected birds. We collected mosquito larvae that were raised in the lab. Adult mosquitoes were divided in 3 groups allowed them to feed overnight on a P. relictum-infected bird, a P. cathemerium-infected bird and an uninfected control bird. For transcriptome analyses, we processed mosquitoes at three time points after exposure, 24 hours post-infection (hpi), 10 days post-infection (dpi) and 21 dpi. At each time point, we created pools of 5 mosquitoes of each infection status capturing the mosquitoes alive and immediately transferring them to dry ice. We preserved the mosquitoes at -80ºC until RNA extractions were carried out. We collected a total of 36 samples including controls (4 pools x 3 time points x 3 conditions). We extracted RNA and DNA from pools of 5 mosquitoes using TRIzol® (Invitrogen, Carlsbad, CA, USA) followed by column purification using RNeasy mini kit® (QIAGEN, Hilden, Germany). RNA samples were submitted to the Polo d’Innovazione di Genomica, Genetica e Biologia, Siena (Italy) where library preparation and sequencing were carried out. [Methods for processing the data] Illumina sequencing and RNAseq pipeline available with the published paper. |
URI: | http://hdl.handle.net/10261/340499 | DOI: | https://doi.org/10.20350/digitalCSIC/15708 | Referencias: | Garrigós Marta, Ylla Guillem, Martínez-de la Puente Josué, Figuerola Jordi, Ruiz-López María José. Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens. Molecular Ecology, 2023. |
| Aparece en las colecciones: | (EBD) Conjuntos de datos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| RNAseq-project.xlsx | 26,99 kB | Microsoft Excel XML | Visualizar/Abrir | |
| README.txt | 5,3 kB | Text | Visualizar/Abrir |
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