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| Título: | Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase |
Autor: | Blanco, Luis CSIC ORCID ; Prieto, Ignacio CSIC ORCID; Gutiérrez, Julio; Bernad, Antonio CSIC ORCID; Lázaro, José M. CSIC; Hermoso, José Miguel; Salas, Margarita CSIC ORCID | Fecha de publicación: | 1-dic-1987 | Editor: | American Society for Microbiology | Citación: | Journal of Virology 61(12): 3983-3991 (1987) | Resumen: | Ammonium ions stimulated the formation of the phi diameter 29 protein p3-dAMP initiation complex by decreasing the Km value for dATP in a purified system containing the viral terminal protein p3, the viral DNA polymerase p2, and the phi 29 DNA-protein p3 complex as a template. In addition, NH4+ ions stimulated the amount of p3-dAMP complex elongation and increased by about twofold the rate of elongation. The stimulatory effect of NH4+ ions on in vitro phi 29 DNA replication is probably related to the formation of a stable complex between the terminal protein and the DNA polymerase, which was detected only in the presence of NH4+ ions. | URI: | http://hdl.handle.net/10261/338655 | Identificadores: | issn: 0022-538X e-issn: 1098-5514 |
| Aparece en las colecciones: | (CBM) Artículos |
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