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Título: | DNA polymerase lambda (Pol λ), a novel eukaryotic DNA polymerase with a potential role in meiosis |
Autor: | García-Díaz, Miguel; Domı́nguez, Orlando; López-Fernández, Luis A. CSIC ORCID; Teresa de Lera, Laı́n; Sanı́ger, Marı́a L.; Ruiz, José F. CSIC ORCID; Párraga, Mario; Garcı́a-Ortiz, Marı́a J.; Kirchhoff, Tomas; Del Mazo, Jesús CSIC ORCID ; Bernad, Antonio CSIC ORCID; Blanco, Luis CSIC ORCID | Palabras clave: | DNA polymerase BRCT domain Pachytene spematocytes Meiotic recombination DNA repair |
Fecha de publicación: | 25-ago-2000 | Editor: | Elsevier | Citación: | Journal of Molecular Biology 301(4): 851-867 (2000) | Resumen: | A new gene (POLL) encoding a novel DNA polymerase (Pol λ) has been identified at mouse chromosome 19. Murine Pol λ, consisting of 573 amino acid residues, has a 32 % identity to Pol β, involved in nuclear DNA repair in eukaryotic cells. It is interesting that Pol λ contains all the critical residues involved in DNA binding, nucleotide binding and selection, and catalysis of DNA polymerization, that are conserved in Pol β and other DNA polymerases belonging to family X. Murine Pol λ, overproduced in Escherichia coli, displayed intrinsic DNA polymerase activity when assessed by in situ gel analysis. Pol λ also conserves the critical residues of Pol β required for its intrinsic deoxyribose phosphate lyase (dRPase) activity. The first 230 amino acid residues of Pol λ, that have no counterpart in Pol β, contain a BRCT domain, present in a variety of cell-cycle check-point control proteins responsive to DNA damage and proteins involved in DNA repair. Northern blotting, in situ hybridization analysis and immunostaining showed high levels of Pol λ specifically expressed in testis, being developmentally regulated and mainly associated to pachytene spermatocytes. These first evidences, although indirect, suggest a potential role of Pol λ in DNA repair synthesis associated with meiosis. | Versión del editor: | http://dx.doi.org/10.1006/jmbi.2000.4005 | URI: | http://hdl.handle.net/10261/338490 | DOI: | 10.1006/jmbi.2000.4005 | Identificadores: | issn: 0022-2836 |
Aparece en las colecciones: | (CBM) Artículos (CIB) Artículos (CNB) Artículos |
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