Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/32735
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Barral, Patricia | - |
dc.contributor.author | Suárez, Cynthia | - |
dc.contributor.author | Batanero, Eva | - |
dc.contributor.author | Alfonso, Carlos | - |
dc.contributor.author | Alché Ramírez, Juan de Dios | - |
dc.contributor.author | Rodríguez García, María I. | - |
dc.contributor.author | Villalba, Mayte | - |
dc.contributor.author | Rivas, Germán | - |
dc.contributor.author | Rodríguez, Rosalía | - |
dc.date.accessioned | 2011-02-23T14:17:33Z | - |
dc.date.available | 2011-02-23T14:17:33Z | - |
dc.date.issued | 2005-08 | - |
dc.identifier.citation | Biochemical Journal 15(1): 77-84 (2005) | es_ES |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://hdl.handle.net/10261/32735 | - |
dc.description | 8 páginas, 8 figuras, 2 tablas. | es_ES |
dc.description.abstract | CBMs (carbohydrate-binding modules) are the most common non-catalytic modules associated with enzymes active in plant cell-wall hydrolysis. They have been frequently identified by amino acid sequence alignments, but only a few have been experimentally established to have a carbohydrate-binding activity. A small olive pollen protein, Ole e 10 (10 kDa), has been described as a major inducer of type I allergy in humans. In the present study, the ability of Ole e 10 to bind several polysaccharides has been analysed by affinity gel electrophoresis, which demonstrated that the protein bound 1,3-b-glucans preferentially. Analytical ultracentrifugation studies confirmed binding to laminarin, at a protein/ligand ratio of 1:1. The interaction of Ole e 10 with laminarin induced a conformational change in the protein, as detected by CD and fluorescence analyses, and an increase of 3.6 °C in the thermal denaturation temperature of Ole e 10 in the presence of the glycan. These results, and the absence of alignment of the sequence of Ole e 10 with that of any classified CBM, indicate that this pollen protein defines a novel family of CBMs, which we propose to name CBM43. Immunolocalization of Ole e 10 in mature and germinating pollen by transmission electron microscopy and confocal laser scanning microscopy demonstrated the co-localization of Ole e 10 and callose (1,3-b-glucan) in the growing pollen tube, suggesting a role for this protein in the metabolism of carbohydrates and in pollen tube wall re-formation during germination. | es_ES |
dc.description.sponsorship | This work was supported by grant SAF2002-02711 from the Dirección General de Investigación (Ministerio de Ciencia y Tecnología, Spain). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Portland Press | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Allergens | es_ES |
dc.subject | Carbohydrate-binding module | es_ES |
dc.subject | 1,3-b-glucan | es_ES |
dc.subject | Olive pollen | es_ES |
dc.subject | Pollen germination | es_ES |
dc.title | An olive pollen protein with allergenic activity, Ole e 10, defines a novel family of carbohydrate-binding modules and is potentially implicated in pollen germination | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1042/BJ20050456 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1042/BJ20050456 | es_ES |
dc.identifier.pmid | 15882149 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (EEZ) Artículos (CIB) Artículos |
CORE Recommender
PubMed Central
Citations
24
checked on 20-abr-2024
SCOPUSTM
Citations
64
checked on 12-may-2024
WEB OF SCIENCETM
Citations
57
checked on 25-feb-2024
Page view(s)
442
checked on 12-may-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.