SAUR63 fusion protein lipid binding

Nagpal, Punita; Reeves, Paul H.; Wong, Jeh Haur; Armengot, Laia; Chae, Keun; Rieveschl, Nathaniel B.; Trinidad, Brendan; Davidsdottir, Vala; Jain, Prateek; Gray, William M.; Jaillais, Yvon; Reed, Jason W.

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Título:	SAUR63 fusion protein lipid binding
Autor:	Nagpal, Punita; Reeves, Paul H.; Wong, Jeh Haur; Armengot, Laia CSIC ORCID; Chae, Keun; Rieveschl, Nathaniel B.; Trinidad, Brendan; Davidsdottir, Vala; Jain, Prateek; Gray, William M.; Jaillais, Yvon; Reed, Jason W.
Palabras clave:	Stimulate plasma membrana Lipid binding assays Dissected structural requirements Decreasing protein level Bypass genetic redundancy Arabidopsis thaliana Pm electrochemical potential Pm anionic lipids Stimulating cell expansion Saur63 fusion protein Div><p Cell expansion Inhiting pm Various organs Thereby increasing Terminal domain Several members Saur63 growth Root cells Promoting activity Increase growth Function approach Eliminating growth D5 phosphatase Antagonize pp2c
Fecha de publicación:	19-sep-2022
Editor:	Figshare
Citación:	Nagpal, Punita; Reeves, Paul H.; Wong, Jeh Haur; Armengot, Laia; Chae, Keun; Rieveschl, Nathaniel B.; Trinidad, Brendan; Davidsdottir, Vala; Jain, Prateek; Gray, William M.; Jaillais, Yvon; Reed, Jason W.; 2022; SAUR63 fusion protein lipid binding [Dataset]; Figshare; https://doi.org/10.1371/journal.pgen.1010375.s015
Resumen:	A) Western blot showing presence of fusion proteins in extracts used in lipid blot experiments in Fig 5A. Arrows indicate locations of full-length SAUR63:YFP:HA and truncated SAUR6326-142:YFP:HA fusion proteins (upper arrow) and SAUR631-25:YFP:HA N-terminal domain fusion protein (lower arrow). B) Longer exposures of two lipid blots from Fig 5A. C) Mock experiment in which extracts were incubated for 70 minutes in protein extraction buffer at the indicated temperatures, and then run on a gel for western blots. For both SAUR63:YFP:HA and SAUR6326-142:YFP:HA, similar amounts of protein are present after incubation at -20 C or after incubation at 22 C, as during the lipid blot binding experiment.
Descripción:	1 figure.
Versión del editor:	https://doi.org/10.1371/journal.pgen.1010375.s015
URI:	http://hdl.handle.net/10261/311200
DOI:	10.1371/journal.pgen.1010375.s015
Referencias:	Nagpal, Punita; Reeves, Paul H.; Wong, Jeh Haur; Armengot, Laia; Chae, Keun; Rieveschl, Nathaniel B.; Trinidad, Brendan; Davidsdottir, Vala; Jain, Prateek; Gray, William M.; Jaillais, Yvon; Reed, Jason W. SAUR63 stimulates cell growth at the plasma membrane. PLoS Genetics 18(9): e1010375 (2022). https://doi.org/10.1371/journal.pgen.1010375. http://hdl.handle.net/10261/303499
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